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Stem cells and calcium phosphate cement scaffolds for bone regeneration
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نویسنده
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wang p. ,zhao l. ,chen w. ,liu x. ,weir m.d. ,xu h.h.k.
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منبع
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journal of dental research - 2014 - دوره : 93 - شماره : 7 - صفحه:618 -625
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چکیده
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Calcium phosphate cements (cpcs) have excellent biocompatibility and osteoconductivity for dental,craniofacial,and orthopedic applications. this article reviews recent developments in stem cell delivery via cpc for bone regeneration. this includes: (1) biofunctionalization of the cpc scaffold,(2) co-culturing of osteoblasts/ endothelial cells and prevascularization of cpc,(3) seeding of cpc with different stem cell species,(4) human umbilical cord mesenchymal stem cell (hucmsc) and bone marrow msc (hbmsc) seeding on cpc for bone regeneration,and (5) human embryonic stem cell (hesc) and induced pluripotent stem cell (hipsc) seeding with cpc for bone regeneration. cells exhibited good attachment/ proliferation in cpc scaffolds. stem-cell-cpc constructs generated more new bone and blood vessels in vivo than did the cpc control without cells. hucmscs,hesc-mscs,and hipsc-mscs in cpc generated new bone and blood vessels similar to those of hbmscs; hence,they were viable cell sources for bone engineering. cpc with hesc-mscs and hipsc-mscs generated new bone two- to three-fold that of the cpc control. therefore,this article demonstrates that: (1) cpc scaffolds are suitable for delivering cells; (2) hucmscs,hescs,and hipscs are promising alternatives to hbmscs,which require invasive procedures to harvest with limited cell quantity; and (3) stem-cell-cpc constructs are highly promising for bone regeneration in dental,craniofacial,and orthopedic applications. © international & american associations for dental research.
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کلیدواژه
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Animal studies; Bone tissue engineering; Calcium phosphate scaffold; Dental and craniofacial; Human embryonic stem cells; Human induced pluripotent stem cells
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آدرس
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biomaterials and tissue engineering division,department of endodontics,prosthodontics and operative dentistry,university of maryland dental school,baltimore,md 21201,united states,state key laboratory of oral diseases,west china hospital of stomatology,sichuan university,chengdu,sichuan 610041, China, biomaterials and tissue engineering division,department of endodontics,prosthodontics and operative dentistry,university of maryland dental school,baltimore,md 21201,united states,department of orthopaedic surgery,nanfang hospital,southern medical university,guangzhou,guangdong 510515, China, biomaterials and tissue engineering division,department of endodontics,prosthodontics and operative dentistry,university of maryland dental school,baltimore,md 21201,united states,state key laboratory of oral diseases,west china hospital of stomatology,sichuan university,chengdu,sichuan 610041, China, biomaterials and tissue engineering division,department of endodontics,prosthodontics and operative dentistry,university of maryland dental school,baltimore,md 21201,united states,state key laboratory of oral diseases,west china hospital of stomatology,sichuan university,chengdu,sichuan 610041, China, biomaterials and tissue engineering division,department of endodontics,prosthodontics and operative dentistry,university of maryland dental school,baltimore,md 21201, United States, biomaterials and tissue engineering division,department of endodontics,prosthodontics and operative dentistry,university of maryland dental school,baltimore,md 21201,united states,mechanical engineering department,university of maryland baltimore county,baltimore,md 21250,united states,biomaterials and tissue engineering division,university of maryland,school of medicine,baltimore,md 21201, United States
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Authors
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