Soft-tissue wound healing by anti-advanced glycation end-products agents

The blocking of advanced glycation end-products (age) has been shown to reduce diabetic complications and control periodontitis. this study investigated the pattern of palatal wound-healing after graft harvesting under the administration of aminoguanidine (ag),an age inhibitor,or n-phenacylthiazolium bromide (ptb),a glycated cross-link breaker. full-thickness palatal excisional wounds (5.0 x 1.5 mm2) were created in 72 sprague-dawley rats. the rats received daily intraperitoneal injections of normal saline (control),ag,or ptb and were euthanized after 4 to 28 days. the wound-healing pattern was assessed by histology,histochemistry for collagen matrix deposition,immunohistochemistry for age and the age receptor (rage),and the expression of rage,as well as inflammation- and recovery-associated genes. in the first 14 days following ag or ptb treatments,wound closure,re-epithelialization,and collagen matrix deposition were accelerated,whereas age deposition,rage-positive cells,and inflammation were reduced. rage and tumor necrosis factor-alpha were significantly down-regulated at day 7,and heme oxygenase-1 was persistently down-regulated until day 14. the levels of vascular endothelial growth factor,periostin,type i collagen,and fibronectin were all increased at day 14. in conclusion,anti-age agents appeared to facilitate palatal wound-healing by reducing age-associated inflammation and promoting the recovery process. © international & american associations for dental research.