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Erythropoietin promotes bone formation through EphrinB2/EphB4 signaling
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نویسنده
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li c. ,shi c. ,kim j. ,chen y. ,ni s. ,jiang l. ,zheng c. ,li d. ,hou j. ,taichman r.s. ,sun h.
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منبع
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journal of dental research - 2015 - دوره : 94 - شماره : 3 - صفحه:455 -463
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چکیده
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Recent studies have demonstrated that erythropoietin (epo) has extensive nonhematopoietic biological functions. however,little is known about how epo regulates bone formation,although several studies suggested that epo can affect bone homeostasis. in this study,we investigated the effects of epo on the communication between osteoclasts and osteoblasts through the ephrinb2/ephb4 signaling pathway. we found that epo slightly promotes osteoblastic differentiation with the increased expression of ephb4 in st2 cells. however,epo increased the expression of nfatc1 and ephrinb2 but decreased the expression of mmp9 in raw264.7 cells,resulting in an increase of ephrinb2-expressing osteoclasts and a decrease in resorption activity. the stimulation of ephrinb2/ephb4 signaling via ephrinb2-fc significantly promoted epo-mediated osteoblastic differentiation in st2 cells. ephb4 knockdown through ephb4 shrna inhibited epo-mediated osteoblastic phenotypes. furthermore,in vivo assays clearly demonstrated that epo efficiently induces new bone formation in the alveolar bone regeneration model. taken together,these results suggest that ephrinb2/ephb4 signaling may play an important role in epo-mediated bone formation. © international & american associations for dental research 2015.
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کلیدواژه
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cytokines; hematopoietic growth factors; hormones; osteoblast; osteogenesis; skeletal
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آدرس
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department of oral pathology,school and hospital of stomatology,jilin university,changchun, China, department of oral pathology,school and hospital of stomatology,jilin university,changchun, China, department of periodontics and oral medicine,university of michigan,school of dentistry,ann arbor,mi, United States, department of stomatology,first people's hospital of chenzhou,chenzhou,hunan province, China, department of oral pathology,school and hospital of stomatology,jilin university,changchun, China, department of oral pathology,school and hospital of stomatology,jilin university,changchun, China, national institute of dental and craniofacial research,national institutes of healthmd, United States, department of oral pathology,school and hospital of stomatology,jilin university,changchun, China, department of oral pathology,school and hospital of stomatology,jilin university,changchun, China, department of periodontics and oral medicine,university of michigan,school of dentistry,ann arbor,mi, United States, department of oral pathology,school and hospital of stomatology,jilin university,changchun, China
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Authors
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