PPAR maintains homeostasis through autophagy regulation in dental pulp

This study investigated the relevance between pulp vitality and autophagy in aged human dental pulp cells (hdpcs) and whether peroxisome proliferator-activated receptor gamma (ppar) affects autophagy regulation for homeostasis in the aging progress. in vivo experiments were used in human and sprague-dawley rat teeth obtained from young and adult individuals. aging- and autophagy-related molecules were determined by immunohistochemistry and hematoxylin and eosin staining. hdpcs were serially subcultured until spontaneously arrested for in vitro aging,and the replication deficiency adenovirus was introduced for ppar overexpression. subsequently,the effect of ppar on regulation of autophagy molecules,mitochondria activity,and cell viability was assessed using western blotting,confocal microscopy,and the mtt assay,respectively. in adult pulp tissue,autophagy molecules (autophagy protein 5,microtubule-associated protein 1a/1b light chain,and beclin-1) were increased,but aging-related (ppar and heme oxygenase 1 [ho-1]) and dentinogenesis (dentin sialophosphoprotein and dentin matrix acidic phosphoprotein) molecules were decreased. in aged hdpcs,autophagy and intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 were increased,while ppar and ho-1 were decreased. under stimulation with lipopolysaccharide,autophagy- and aging-related molecules were differentially expressed between young and aged cells. ppar induced ho-1 and autophagy molecules but reduced inflammatory molecules in aged cells. in addition,ppar activated strong mitochondrial activity and cell viability in aging cells. inhibition of ho-1 by tin protoporphyrin ix exacerbated autophagy and mitochondrial activity as well as cell viability in young cells. this study indicates that ppar maintains pulp homeostasis through the regulation of autophagy molecules during the life span of hdpcs. © 2015 international & american associations for dental research.