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Effects of hypoxia on the immunomodulatory properties of human gingiva-derived mesenchymal stem cells
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نویسنده
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jiang c.m. ,liu j. ,zhao j.y. ,xiao l. ,an s. ,gou y.c. ,quan h.x. ,cheng q. ,zhang y.l. ,he w. ,wang y.t. ,yu w.j. ,huang y.f. ,yi y.t. ,chen y. ,wang j.
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منبع
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journal of dental research - 2015 - دوره : 94 - شماره : 1 - صفحه:69 -77
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چکیده
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The environment of bone marrow mesenchymal stem cells (mscs) is hypoxic,which plays an important role in maintaining their self-renewal potential and undifferentiated state. mscs have been proven to possess immunomodulatory properties and have been used clinically to treat autoimmune diseases. here,we tested the effects of hypoxia on the immunomodulatory properties of mscs and examined its possible underlying mechanisms. we found that hypoxic stimulation promoted the immunomodulatory properties of human gingiva-derived mesenchymal stem cells (hgmscs) by enhancing the suppressive effects of hgmscs on peripheral blood mononuclear cells (pbmcs). the proliferation of pbmcs was significantly inhibited,while the apoptosis of pbmcs was increased,which was associated with the fas ligand (fasl) expression of hgmscs. the in vivo study showed that systemically infused hgmscs could enhance skin wound repair,and 24-h hypoxic stimulation significantly promoted the reparative capacity of hgmscs. for mechanism,hgmsc treatment inhibited the local inflammation of injured skin by suppressing the inflammatory cells,reducing the pro-inflammatory cytokine tumor necrosis factor-î± (tnf-î±),and increasing anti-inflammatory cytokine interleukin-10 (il-10),which was promoted by hypoxia. hypoxia preconditioning may be a good optimizing method to promote the potential of mscs for the future cell-based therapy. © international & american associations for dental research 2014.
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کلیدواژه
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apoptosis; cell proliferation; Fas ligand protein; immunomodulation; interleukin-10; oxygen
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آدرس
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department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, laboratory of stem cell biology,state key laboratory of biotherapy,sichuan university,chengdu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, qingdao first sanatorium of jinan military distract of pla,qingdao shandong, China, department of orthodontics,luzhou medical college,luzhou,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China, department of orthodontics,west china hospital of stomatology,sichuan university,people s south road,chendu,sichuan, China
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Authors
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