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   Three-dimensional imaging reveals new compartments and structural adaptations in odontoblasts  
   
نویسنده khatibi shahidi m. ,krivanek j. ,kaukua n. ,ernfors p. ,hladik l. ,kostal v. ,masich s. ,hampl a. ,chubanov v. ,gudermann t. ,romanov r.a. ,harkany t. ,adameyko i. ,fried k.
منبع journal of dental research - 2015 - دوره : 94 - شماره : 7 - صفحه:945 -954
چکیده    In organized tissues,the precise geometry and the overall shape are critical for the specialized functions that the cells carry out. odontoblasts are major matrix-producing cells of the tooth and have also been suggested to participate in sensory transmission. however,refined morphologic data on these important cells are limited,which hampers the analysis and understanding of their cellular functions. we took advantage of fluorescent color-coding genetic tracing to visualize and reconstruct in 3 dimensions single odontoblasts,pulp cells,and their assemblages. our results show distinct structural features and compartments of odontoblasts at different stages of maturation,with regard to overall cellular shape,formation of the main process,orientation,and matrix deposition. we demonstrate previously unanticipated contacts between the processes of pulp cells and odontoblasts. all reported data are related to mouse incisor tooth. we also show that odontoblasts express trpm5 and piezo2 ion channels. piezo2 is expressed ubiquitously,while trpm5 is asymmetrically distributed with distinct localization to regions proximal to and within odontoblast processes. © international & american associations for dental research 2015.
کلیدواژه cell morphology; dentinogenesis; growth/development; protein expression; pulp biology; transgenic animals
آدرس department of dental medicine,karolinska institutet,huddinge, Sweden, department of histology and embryology,faculty of medicine,masaryk university,brno, Czech Republic, department of neuroscience,karolinska institutet,retzius vag 8,stockholm, Sweden, unit of molecular neurobiology,department of medical biochemistry and biophysics,karolinska institutet,stockholm, Sweden, tescan orsay holding,brno, Czech Republic, tescan orsay holding,brno, Czech Republic, department of cell and molecular biology,karolinska institutet,stockholm, Sweden, department of histology and embryology,faculty of medicine,masaryk university,brno, Czech Republic, ludwig-maximilians-universität münchen,walther-straub-institut für pharmakologie und toxikologie,münchen, Germany, ludwig-maximilians-universität münchen,walther-straub-institut für pharmakologie und toxikologie,münchen, Germany, unit of molecular neurobiology,department of medical biochemistry and biophysics,karolinska institutet,stockholm, Sweden, unit of molecular neurobiology,department of medical biochemistry and biophysics,karolinska institutet,stockholm,sweden,department of molecular neurosciences,center of brain research,medical university of vienna,vienna, Austria, department of molecular neurosciences,center of brain research,medical university of vienna,vienna,austria,department of physiology and pharmacology,karolinska institutet,stockholm, Sweden, department of neuroscience,karolinska institutet,retzius vag 8,stockholm, Sweden
 
     
   
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