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Plap-1/asporin positively regulates FGF-2 activity
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نویسنده
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awata t. ,yamada s. ,tsushima k. ,sakashita h. ,yamaba s. ,kajikawa t. ,yamashita m. ,takedachi m. ,yanagita m. ,kitamura m. ,murakami s.
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منبع
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journal of dental research - 2015 - دوره : 94 - شماره : 10 - صفحه:1417 -1424
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چکیده
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Plap-1 is an extracellular matrix protein that is predominantly expressed in the periodontal ligament within periodontal tissue. it was previously revealed that plap-1 negatively regulates bone morphogenetic protein 2 and transforming growth factor β activity through direct interactions. however,the interaction between plap-1 and other growth factors has not been defined. here,we revealed that plap-1 positively regulates the activity of fibroblast growth factor 2 (fgf-2),a critical growth factor in tissue homeostasis and repair. in this study,we isolated mouse embryonic fibroblasts (mefs) from plap-1-/- mice generated in our laboratory. interestingly,plap-1-/- mefs exhibited enhanced responses to bone morphogenetic protein 2 but defective responses to fgf-2,and plap-1 transfection into plap-1-/- mefs rescued these defective responses. in addition,binding assays revealed that plap-1 promotes fgf-2-fgf receptor 1 (fgfr1) complex formation by direct binding to fgf-2. immunocytochemistry analyses revealed colocalization of plap-1 and fgf-2 in wild-type mefs and reduced colocalization of fgf-2 and fgfr1 in plap-1-/- mefs compared with wild-type mefs. taken together,plap-1 positively regulates fgf-2 activity through a direct interaction. extracellular matrix-growth factor interactions have considerable effects; thus,this approach may be useful in several regenerative medicine applications. © international & american associations for dental research 2015.
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کلیدواژه
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BMP-2; Cell differentiation; Cell proliferation; Extracellular matrix; Growth factors; Periodontal ligament
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آدرس
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department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan, department of periodontology,osaka university,graduate school of dentistry,1-8 yamadaoka,suita,osaka, Japan
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Authors
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