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Sclerostin Deficiency Promotes Reparative Dentinogenesis
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نویسنده
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collignon a.-m. ,amri n. ,lesieur j. ,sadoine j. ,ribes s. ,menashi s. ,simon s. ,berdal a. ,rochefort g.y. ,chaussain c. ,gaucher c.
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منبع
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journal of dental research - 2017 - دوره : 96 - شماره : 7 - صفحه:815 -821
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چکیده
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In humans,the sost gene encodes sclerostin,an inhibitor of bone growth and remodeling,which also negatively regulates the bone repair process. sclerostin has also been implicated in tooth formation,but its potential role in pulp healing remains unknown. the aim of this study was to explore the role of sclerostin in reparative dentinogenesis using sost knockout mice (sost-/-). the pulps of the first maxillary molars were mechanically exposed in 3-mo-old sost-/- and wild-type (wt) mice (n = 14 mice per group),capped with mineral trioxide aggregate cement,and the cavities were filled with a bonded composite resin. reparative dentinogenesis was dynamically followed up by micro-computed tomography and characterized by histological analyses. presurgical analysis revealed a significantly lower pulp volume in sost-/- mice compared with wt. at 30 and 49 d postsurgery,a large-forming reparative mineralized bridge,associated with osteopontin-positive mineralization foci,was observed in the sost-/- pulps,whereas a much smaller bridge was detected in wt. at the longer time points,the bridge,which was associated with dentin sialoprotein-positive cells,had expanded in both groups but remained significantly larger in sost-/- pulps. sclerostin expression in the healing wt pulps was detected in the cells neighboring the forming dentin bridge. in vitro,mineralization induced by sost-/- dental pulp cells (dpcs) was also dramatically enhanced when compared with wt dpcs. these observations were associated with an increased sost expression in wt cells. taken together,our data show that sclerostin deficiency hastened reparative dentinogenesis after pulp injury,suggesting that the inhibition of sclerostin may constitute a promising therapeutic strategy for improving the healing of damaged pulps. © international & american associations for dental research.
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کلیدواژه
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dental pulp cells; mineralization; odontoblast; pulp injury model; Sost gene; tooth dentin
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آدرس
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ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge,france,departments of odontology,univ. hospitals louis mourier and bretonneau natl. rare dis. ctr. metabolism phosphorus and calcium,rothschild national rare diseases center maface (huep),paris, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge,france,inserm umrs 1138,molecular oral pathophysiology team,paris diderot and paris descartes university uspc,paris, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge, France, departments of odontology,univ. hospitals louis mourier and bretonneau natl. rare dis. ctr. metabolism phosphorus and calcium,rothschild national rare diseases center maface (huep),paris,france,inserm umrs 1138,molecular oral pathophysiology team,paris diderot and paris descartes university uspc,paris, France, departments of odontology,univ. hospitals louis mourier and bretonneau natl. rare dis. ctr. metabolism phosphorus and calcium,rothschild national rare diseases center maface (huep),paris,france,inserm umrs 1138,molecular oral pathophysiology team,paris diderot and paris descartes university uspc,paris, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge,france,departments of odontology,univ. hospitals louis mourier and bretonneau natl. rare dis. ctr. metabolism phosphorus and calcium,rothschild national rare diseases center maface (huep),paris, France, ea 2496 laboratory orofacial pathologies,imagery and biotherapies,university paris descartes sorbonne paris cité,1 rue maurice arnoux,montrouge,france,departments of odontology,univ. hospitals louis mourier and bretonneau natl. rare dis. ctr. metabolism phosphorus and calcium,rothschild national rare diseases center maface (huep),paris, France
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Authors
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