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MiR-146a induces differentiation of periodontal ligament cells
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نویسنده
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hung p.-s. ,chen f.-c. ,kuang s.-h. ,kao s.-y. ,lin s.-c. ,chang k.-w.
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منبع
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journal of dental research - 2010 - دوره : 89 - شماره : 3 - صفحه:252 -257
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چکیده
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Differentiation of periodontal ligament (pdl) cells occurs under specific induction; furthermore,nf-κb signaling is important for regulation of bone differentiation. micrornas are small non-coding rnas that repress the translation of target genes and modulate cellular processes. mir-146a has been reported to modulate nf-κb signaling. this study hypothesized that mir-146a has a regulatory role in pdl differentiation by affecting nf-κb signaling. immortalized pdl (i-pdl) cell lines were established by exogenous telomerase expression. the genesis of alkaline phosphatase and the up-regulation of mir-146a were induced by ascorbic acid in the i-pdl cells and primary pdl cells. i-pdl cells with exogenous mir-146a expression showed attenuation of nf-κb activity and exhibited higher differentiation relative to the controls. exogenous nf-κb expression decreased the expression of differentiation markers,while the inactivation of endogenous nf-κb increased alkaline phosphatase in i-pdl cells. this study concludes that mir-146a promotes the differentiation in pdl cells through the down-regulation of nf-κb signaling.
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کلیدواژه
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Differentiation; MicroRNA; MiR-146a; NF-κB.; Periodontal ligament
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آدرس
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institute of oral biology,school of dentistry,national yang-ming university,no. 155,li-nong st.,taipei 112, Taiwan, department of dentistry,school of dentistry,national yang-ming university,no. 155,li-nong st.,taipei 112,taiwan,department of stomatology,taipei veterans general hospital,taipei, Taiwan, department of dentistry,school of dentistry,national yang-ming university,no. 155,li-nong st.,taipei 112,taiwan,department of stomatology,taipei veterans general hospital,taipei, Taiwan, department of dentistry,school of dentistry,national yang-ming university,no. 155,li-nong st.,taipei 112,taiwan,department of stomatology,taipei veterans general hospital,taipei, Taiwan, institute of oral biology,school of dentistry,national yang-ming university,no. 155,li-nong st.,taipei 112, Taiwan, department of dentistry,school of dentistry,national yang-ming university,no. 155,li-nong st.,taipei 112,taiwan,medical education and research,taipei veterans general hospital,taipei, Taiwan
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Authors
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