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MAPK signaling is required for LPS-induced VEGF in pulp stem cells
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نویسنده
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botero t.m. ,son j.s. ,vodopyanov d. ,hasegawa m. ,shelburne c.e. ,nör j.e.
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منبع
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journal of dental research - 2010 - دوره : 89 - شماره : 3 - صفحه:264 -269
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چکیده
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Caries-induced pulpitis is typically accompanied by an increase in dental pulp microvascular density. however,the mechanisms by which dental pulp cells recognize lipopolysaccharides (lpss) remain unclear. we hypothesized that porphyromonas endodontalis and escherichia coli lpss induce vascular endothelial growth factor (vegf) expression in dental pulp stem cells (dpsc) and human dental pulp fibroblasts (hdpf) through mitogen-activated protein kinase (mapk) signaling. elisa,semi-quantitative rt-pcr,immunofluorescence,and western blots were used. here,we observed that lpss induced vegf expression in dpsc and hdpf cells,and both cell types express toll-like receptor 4 (tlr-4). notably,lps-induced vegf is associated with phosphorylation of protein kinase c (pkc ζ) and extracellular signal-regulator kinase (erk1/2) and is dependent upon mapk activation. analysis of these data,collectively,unveils a signaling pathway responsible for synthesis of vegf by pulp cells and suggests a novel therapeutic target for the management of vascular responses in teeth with pulpitis.
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کلیدواژه
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DPSC; LPS; MAPK signaling; TLR-4; VEGF
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آدرس
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department of cariology,restorative sciences,and endodontics,university of michigan,school of dentistry,1011 n. university,ann arbor,mi 48109-1078, United States, department of cariology,restorative sciences,and endodontics,university of michigan,school of dentistry,1011 n. university,ann arbor,mi 48109-1078, United States, department of cariology,restorative sciences,and endodontics,university of michigan,school of dentistry,1011 n. university,ann arbor,mi 48109-1078, United States, department of pathology,university of michigan,medical school, United States, department of biology and materials sciences,university of michigan,school of dentistry, United States, department of cariology,restorative sciences,and endodontics,university of michigan,school of dentistry,1011 n. university,ann arbor,mi 48109-1078,united states,department of biomedical engineering,university of michigan college of engineering,united states,university of michigan comprehensive cancer center,ann arbor,mi 48109, United States
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Authors
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