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Inorganic polyphosphate induces osteoblastic differentiation
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نویسنده
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usui y. ,uematsu t. ,uchihashi t. ,takahashi m. ,takahashi m. ,ishizuka m. ,doto r. ,tanaka h. ,komazaki y. ,osawa m. ,yamada k. ,yamaoka m. ,furusawa k.
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منبع
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journal of dental research - 2010 - دوره : 89 - شماره : 5 - صفحه:504 -509
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چکیده
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Inorganic polyphosphate [poly(p)] is especially prevalent in osteoblasts. we tested the hypothesis that poly(p) stimulates osteoblastic differentiation and polyphosphate metabolism for bone formation. the osteoblast-like cell line,mc 3t3-e1,was cultured with poly(p),and gene expression was evaluated by real-time reverse-transcription polymerase chain-reaction. phosphatase activity and extracellular matrix mineralization were also determined. the role of poly(p) was assessed in a beagle dog alveolar bone regeneration model. poly(p) increased osteocalcin,osterix,bone sialoprotein,and tissue non-specific alkaline phosphatase gene expression,with a high level of end-polyphosphatase activity,resulting in low-chain-length poly(p),inorganic pyrophosphate,and inorganic phosphate production. mc3t3-e1 cells differentiated into mature osteoblasts and showed expression of ectonucleotide pyrophosphatase phosphodiesterase 1,while mouse progressive ankylosis gene expression remained unchanged. promotion of alveolar bone regeneration was observed in poly(p)-treated beagle dogs. these findings suggest that poly(p) induces osteoblastic differentiation and bone mineralization,and acts as a resource for mineralization.
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کلیدواژه
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Differentiation; Inorganic polyphosphate; Osteoblast; Polyphosphatase.
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آدرس
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department of orthodontics,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, institute for oral science,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874,japan,oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, department of orthodontics,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, department of orthodontics,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, department of orthodontics,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874,japan,institute for oral science,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, institute for oral science,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874,japan,oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan, institute for oral science,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874,japan,oral and maxillofacial surgery,matsumoto dental university,school of dentistry,1780 gobara hirooka,shiojiri,nagano 399-0874, Japan
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Authors
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