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Dental pulp cells for induced pluripotent stem cell banking
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نویسنده
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tamaoki n. ,takahashi k. ,tanaka t. ,ichisaka t. ,aoki h. ,takeda-kawaguchi t. ,iida k. ,kunisada t. ,shibata t. ,yamanaka s. ,tezuka k.
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منبع
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journal of dental research - 2010 - دوره : 89 - شماره : 8 - صفحه:773 -778
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چکیده
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Defined sets of transcriptional factors can reprogram human somatic cells to induced pluripotent stem (ips) cells. however,many types of human cells are not easily accessible to minimally invasive procedures. here we evaluated dental pulp cells (dpcs) as an optimal source of ips cells,since they are easily obtained from extracted teeth and can be expanded under simple culture conditions. from all 6 dpc lines tested with the conventional 3 or 4 reprogramming factors,ips cells were effectively established from 5 dpc lines. furthermore,determination of the hla types of 107 dpc lines revealed 2 lines homozygous for all 3 hla loci and showed that if an ips bank is established from these initial pools,the bank will cover approximately 20% of the japanese population with a perfect match. analysis of these data demonstrates the promising potential of dpc collections as a source of ips cell banks for use in regenerative medicine. © international & american associations for dental research.
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کلیدواژه
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dental pulp cells; HLA types; induced pluripotent stem cells; iPS cell bank
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آدرس
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department of oral and maxillofacial science,gifu university,graduate school of medicine,1-1 yanagido,gifu city,gifu 501-1194, Japan, center for ips cell research and application,institute for integrated cell- material sciences,kyoto university,kyoto 6008813,japan,yamanaka ips cell special project,japan science and technology agency,kawaguchi 3320012, Japan, department of pediatrics,graduate school of medicine,kyoto university,kyoto 6068507, Japan, department of stem cell biology,institute for frontier medical sciences,kyoto university,kyoto 6068507, Japan, department of tissue and organ development,gifu university,graduate school of medicine,11 yanagido,gifu city,gifu 501 1194, Japan, department of oral and maxillofacial science,gifu university,graduate school of medicine,1-1 yanagido,gifu city,gifu 501-1194, Japan, department of oral and maxillofacial science,gifu university,graduate school of medicine,1-1 yanagido,gifu city,gifu 501-1194, Japan, department of tissue and organ development,gifu university,graduate school of medicine,11 yanagido,gifu city,gifu 501 1194, Japan, department of oral and maxillofacial science,gifu university,graduate school of medicine,1-1 yanagido,gifu city,gifu 501-1194, Japan, center for ips cell research and application,institute for integrated cell- material sciences,kyoto university,kyoto 6008813,japan,yamanaka ips cell special project,japan science and technology agency,kawaguchi 3320012,japan,department of pediatrics,graduate school of medicine,kyoto university,kyoto 6068507, Japan, department of oral and maxillofacial science,gifu university,graduate school of medicine,1-1 yanagido,gifu city,gifu 501-1194, Japan
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Authors
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