Efficient cell-seeding into scaffolds improves bone formation

Bone marrow stromal cells (bmscs)/β-tricalcium phosphate (β-tcp) composites have attracted a great deal of attention in bone tissue engineering. if more effective bone regeneration is to be achieved,efficient cell-seeding systems need to be clarified. in this study,we investigated the number of cells contained in composites,and the in vitro/ vivo osteogenic differentiation capacity of composites using 4 conventional systems of seeding rat bmscs into β-tcp: soak,low-pressure,pipette,and syringe systems. the highest number of cells was contained in the composites from the syringe group. moreover,after two-week osteogenic induction in vitro,the composites in the syringe group exhibited the highest osteogenic potential,which continued at 8 weeks after subcutaneous implantation in vivo. our results indicated that efficient and appropriate cell-seeding could improve in vitro/vivo bone formation in composites and thus make a potential clinical contribution to successful bone tissue engineering. abbreviations: bmscs,bone marrow stromal cells; β-tcp,β-tricalcium phosphate; s-d,sprague-dawley; kpa,kilopascal; alp,alkaline phosphatase; n,newton; dna,deoxyribonucleic acid; ocn,osteocalcin; anova,analysis of variance; plsd,protected least-significant difference; and he,hematoxylin and eosin. © international & american associations for dental research.