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Analyzing endodontic infections by deep coverage pyrosequencing
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نویسنده
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li l. ,hsiao w.w.l. ,nandakumar r. ,barbuto s.m. ,mongodin e.f. ,paster b.j. ,fraser-liggett c.m. ,fouad a.f.
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منبع
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journal of dental research - 2010 - دوره : 89 - شماره : 9 - صفحه:980 -984
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چکیده
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Bacterial diversity in endodontic infections has not been sufficiently studied. the use of modern pyrosequencing technology should allow for more comprehensive analysis than traditional sanger sequencing. this study investigated bacterial diversity in endodontic infections through taxonomic classification based on 16s rrna gene sequences generated by 454 gs-flx pyrosequencing and conventional sanger capillary sequencing technologies. sequencings were performed on 7 specimens from endodontic infections. on average,47 vs. 28,590 sequences were obtained per sample for sanger sequencing vs. pyrosequencing,representing a 600-fold difference in ĝ€depth-of- coverage. based on ribosomal database project (rdp ii) classifier analysis,pyrosequencing identified 179 bacterial genera in 13 phyla,which was significantly more than sanger sequencing. the phylum bacteroidetes was the most prevalent bacterial phylum. these results indicate that bacterial communities in endodontic infections are more diverse than previously demonstrated. in addition,deep-coverage pyrosequencing of the 16s rrna gene revealed low-abundance micro-organisms with potential clinical implications. © international & american associations for dental research.
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کلیدواژه
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16S rRNA gene; bacterial diversity; endodontic infection; pyrosequencing; taxonomy
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آدرس
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department of endodontics,prosthodontics and operative dentistry,dental school,university of maryland,650 w. baltimore st.,baltimore,md 21201, United States, institute for genome science,university of maryland,801 w. baltimore st.,biopark,baltimore,md 21201, United States, university of nebraska,lincoln, United States, forsyth institute,140 the fenway,boston,ma 02115, United States, institute for genome science,university of maryland,801 w. baltimore st.,biopark,baltimore,md 21201, United States, forsyth institute,140 the fenway,boston,ma 02115, United States, institute for genome science,university of maryland,801 w. baltimore st.,biopark,baltimore,md 21201, United States, department of endodontics,prosthodontics and operative dentistry,dental school,university of maryland,650 w. baltimore st.,baltimore,md 21201, United States
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Authors
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