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Histatin 1 resists proteolytic degradation when adsorbed to hydroxyapatite
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نویسنده
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mcdonald e.e. ,goldberg h.a. ,tabbara n. ,mendes f.m. ,siqueira w.l.
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منبع
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journal of dental research - 2011 - دوره : 90 - شماره : 2 - صفحه:268 -272
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چکیده
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Histatins are salivary proteins that exhibit a high affinity for hydroxyapatite and contribute to the acquired enamel pellicle. previous studies have observed that,despite the high proteolytic activity in saliva,significant numbers of histatin molecules in acquired enamel pellicle are intact. our working hypothesis was that histatins are less susceptible to proteinases present in saliva when adsorbed on the hydroxyapatite. to test this premise,we incubated histatin 1 with hydroxyapatite and human whole saliva. proteolytic products of this incubation were then characterized by page,hplc,and mass spectrometry. this study shows for the first time that binding to hydroxyapatite confers intact histatin 1 with resistance to proteolytic degradation. © international & american associations for dental research.
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کلیدواژه
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acquired enamel pellicle; histatins; hydroxyapatite; mass spectrometry.; proteolysis; proteomics; saliva
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آدرس
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school of dentistry,schulich school of medicine and dentistry,university of western ontario,london,on n6a 5c1, Canada, school of dentistry,schulich school of medicine and dentistry,university of western ontario,london,on n6a 5c1,canada,department of biochemistry,schulich school of medicine and dentistry,university of western ontario,london,on, Canada, department of biochemistry,schulich school of medicine and dentistry,university of western ontario,london,on, Canada, department of pediatric dentistry,faculty of dentistry,university of são paulo, Brazil, school of dentistry,schulich school of medicine and dentistry,university of western ontario,london,on n6a 5c1,canada,department of biochemistry,schulich school of medicine and dentistry,university of western ontario,london,on, Canada
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Authors
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