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Genetic diversity of plaque mutans streptococci with rep-PCR
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نویسنده
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cheon k. ,moser s.a. ,whiddon j. ,osgood r.c. ,momeni s. ,ruby j.d. ,cutter g.r. ,allison d.b. ,childers n.k.
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منبع
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journal of dental research - 2011 - دوره : 90 - شماره : 3 - صفحه:331 -335
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چکیده
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Mutans streptococci (ms) are key organisms associated with the etiology of dental caries. using probabilities that were tested by oversampling,we designed this study to determine the minimal number of ms isolates from an individual required to evaluate diversity of genotypes. ms isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-pcr). analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (n = 12) and adults (n = 10),respectively. in a follow-up study,reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. a mean of 1.5 genotypes was found in 35 children and 10 adults. these findings provide evidence for the design of studies of ms genotyping that can serve as a model for the analysis of genotypes within individuals. © international & american associations for dental research.
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کلیدواژه
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biostatistics; caries; genotypes; microbial genetics; plaque/plaquebiofilms; Streptococcus mutans
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آدرس
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department of pediatric dentistry,university of alabama school of dentistry,1919 7th avenue south,birmingham,al 35294, United States, department of pathology,university of alabama school of medicine,birmingham,al, United States, department of pathology,university of alabama school of medicine,birmingham,al, United States, department of biological and medical sciences,rochester institute of technology,rochester,ny, United States, department of pediatric dentistry,university of alabama school of dentistry,1919 7th avenue south,birmingham,al 35294, United States, department of pediatric dentistry,university of alabama school of dentistry,1919 7th avenue south,birmingham,al 35294, United States, department of biostatistics,university of alabama at birmingham school of public health,birmingham,al, United States, department of biostatistics,university of alabama at birmingham school of public health,birmingham,al, United States, department of pediatric dentistry,university of alabama school of dentistry,1919 7th avenue south,birmingham,al 35294, United States
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Authors
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