Effects of MMP inhibitors incorporated within dental adhesives

Matrix metalloproteinase (mmp) inhibition has been shown to reduce adhesive bond degradation when applied as a pre-conditioner,adding to clinical steps in the placement of adhesives,but their incorporation within dental adhesives has not been fully explored. this study examined the effect of including 2 mmp inhibitors (bb94 and gm6001) within the primers of 3 commercially available adhesives. fluorometric assay and zymography showed that adhesives with mmp inhibitors had high affinity toward both synthetic fluorogenic fret peptides (95%) and dentin powder substrates,respectively. the immediate microtensile bond strength was enhanced for 2 types of adhesives following the addition of both inhibitors. however,no changes were detected between the control and the inhibitor groups following 3-month storage. the modified two-step etch-and-rinse and single-step systems showed less rhodamine b penetration to the hybrid layer and to the adhesive