Formation of engineered bone with adipose stromal cells from buccal fat pad

A robust method for inducing bone formation from adipose-derived stromal cells (adscs) has not been established. moreover,the efficacy of strong osteogenic inducers including bmp-2 for adsc-mediated bone engineering remains controversial. meanwhile,the buccal fat pad (bfp),which is found in the oral cavity as an adipose-encapsulated mass,has been shown to have potential as a new accessible source of adscs for oral surgeons. however,to date,there have been no reports that define the practical usefulness of adscs from bfp (b-adscs) for bone engineering. here,we report an efficient method of generating bone from b-adscs using rhbmp-2. the analyses show that b-adscs can differentiate in vitro toward the osteoblastic lineage by the addition of rhbmp-2 to culture medium,regardless of the presence of osteoinductive reagents (osr),as demonstrated by measurements of alp activity,in vitro calcification,and osteogenic gene expression. interestingly,adipogenic genes were clearly detectable only in cultures with rhbmp-2 and osr. however,in vivo bone formation was most substantial when b-adscs cultured in this condition were transplanted. thus,b-adscs reliably formed engineered bone when pre-treated with rhbmp-2 for inducing mature osteoblastic differentiation. this study supports the potential translation for b-adsc use in the clinical treatment of bone defects. © 2012 international & american associations for dental research.