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Dentin regeneration using deciduous pulp stem/progenitor cells
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نویسنده
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zheng y. ,wang x.y. ,wang y.m. ,liu x.y. ,zhang c.m. ,hou b.x. ,wang s.l.
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منبع
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journal of dental research - 2012 - دوره : 91 - شماره : 7 - صفحه:676 -682
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چکیده
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Reparative dentin formation is essential for maintaining the integrity of dentin structure during disease or trauma. in this study,we investigated stem/progenitor cell-based tissue engineering for dentin regeneration in a large animal model. porcine deciduous pulp stem/progenitor cells (pdpscs) were mixed with a beta-tricalcium phosphate (β-tcp) scaffold for dentin regeneration. different concentrations of pdpscs were tested to determine the optimal density for dentin regeneration. aliquots of 5×105 pdpscs in 1 ml resulted in the highest number of cells attached to the scaffold and the greatest alkaline phosphatase activity. we labeled pdpscs with green fluorescent protein (gfp) and used the optimal cell numbers mixed with β-tcp to repair pulp chamber roof defects in the premolars of swine. four weeks after transplantation,gfp-positive pdpscs were observed in pdpsc-embedded scaffold constructs. at 16 weeks after transplantation,the pdpscs mixed with β-tcp significantly regenerated the dentin-like structures and nearly completely restored the pulp chamber roof defects. this study demonstrated that the pdpsc/scaffold construct was useful in direct pulp-capping and provides pre-clinical evidence for stem/progenitor cell-based dentin regeneration. © 2012 international & american associations for dental research.
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کلیدواژه
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dental pulp-capping; miniature pig; reparative dentin; stem/progenitor cell therapy; tissue engineering; vital pulp treatment
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آدرس
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department of endodontics,molecular laboratory for gene therapy and tooth regeneration,beijing key laboratory of tooth regeneration and function reconstruction,china,salivary gland disease center,molecular laboratory for gene therapy and tooth regeneration,beijing key laboratory of tooth regeneration and function reconstruction, China, department of endodontics,molecular laboratory for gene therapy and tooth regeneration,beijing key laboratory of tooth regeneration and function reconstruction, China, department of pathology,capital medical university school of stomatology,tian tan xi li no.4,beijing 100050, China, department of pathology,capital medical university school of stomatology,tian tan xi li no.4,beijing 100050, China, salivary gland disease center,molecular laboratory for gene therapy and tooth regeneration,beijing key laboratory of tooth regeneration and function reconstruction, China, department of endodontics,molecular laboratory for gene therapy and tooth regeneration,beijing key laboratory of tooth regeneration and function reconstruction, China, salivary gland disease center,molecular laboratory for gene therapy and tooth regeneration,beijing key laboratory of tooth regeneration and function reconstruction,china,department of biochemistry and molecular biology,capital medical university,school of basic medical sciences,beijing 100069, China
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Authors
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