Microbiological characterization in children with aggressive periodontitis

The objective of this study was to characterize the subgingival microbiota of african-american children with localized aggressive periodontitis (lap). fifty-one children were included. subgingival plaque samples were taken from diseased (dd) and healthy sites (dh) in lap and from healthy sites in hs and hc and analyzed by 16s rrna-based microarrays. aggregatibacter actinomycetemcomitans (aa) was the only species found to be both more prevalent (or = 8.3,p = 0.0025) and abundant (p < 0.01) in dd. filifactor alocis (fa) was also found to be more prevalent in dd (or 2.31,ci 1.06-5.01,p = 0.03). most prevalent species in healthy sites were selenomonas spp,veillonella spp,streptococcus spp,bergeyella sp,and kingella oralis. overall,streptococcus spp,campylobacter gracilis,capnocytophaga granulosa,haemophilus parainfluenzae,and lautropia mirabilis were most abundant in healthy children,while aa,fa,tannerella sp,solobacterium moorei,parvimonas micra,and capnocytophaga sp were most abundant in lap. based on a comprehensive analysis with 16s rrna-based microarrays,aa was strongly associated and site-specific in lap. in contrast,other species were found to be associated with healthy sites and individuals (clinicaltrials.gov number ct01330719).abbreviations: healthy site in healthy sibling (hs); healthy site in healthy control child (hc). © 2012 international & american associations for dental research.