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Endothelial differentiation of SHED requires MEK1/ERK signaling
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نویسنده
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bento l.w. ,zhang z. ,imai a. ,nör f. ,dong z. ,shi s. ,araujo f.b. ,nör j.e.
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منبع
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journal of dental research - 2013 - دوره : 92 - شماره : 1 - صفحه:51 -57
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چکیده
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The discovery that dental pulp stem cells are capable of differentiating into endothelial cells raises the exciting possibility that these cells can be a single source of odontoblasts and vascular networks in dental tissue engineering. the purpose of this study was to begin to define signaling pathways that regulate endothelial differentiation of shed. stem cells from exfoliated deciduous teeth (shed) exposed to endothelial growth medium (egm-2mv) supplemented with vascular endothelial growth factor (vegf) differentiated into vegfr2-positive and cd31-positive endothelial cells in vitro. in vivo,vegfr1-silenced shed seeded in tooth slice/ scaffolds and transplanted into immunodeficient mice showed a reduction in human cd31-positive blood vessels as compared with controls (p = 0.02). exposure of shed to egm2-mv supplemented with vegf induced potent activation of erk and akt signaling,while it inhibited phosphorylation of stat3. notably,genetic (mek1 silencing) or chemical (u0126) inhibition of erk signaling restored constitutive stat3 phosphorylation and inhibited the differentiation of shed into endothelial cells. collectively,analysis of these data unveiled the vegf/mek1/erk signaling pathway as a key regulator of the endothelial differentiation of dental pulp stem cells. © 2013 international & american associations for dental research.
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کلیدواژه
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angiogenesis; blood vessels; dental pulp stem cells; endodontics; stemness; tissue engineering
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آدرس
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angiogenesis research laboratory,department of restorative sciences,university of michigan school of dentistry,1011 n. university,ann arbor,mi 48109-1078,united states,department of pediatric dentistry,school of dentistry,federal university of rio grande do sul, Brazil, angiogenesis research laboratory,department of restorative sciences,university of michigan school of dentistry,1011 n. university,ann arbor,mi 48109-1078, United States, angiogenesis research laboratory,department of restorative sciences,university of michigan school of dentistry,1011 n. university,ann arbor,mi 48109-1078, United States, angiogenesis research laboratory,department of restorative sciences,university of michigan school of dentistry,1011 n. university,ann arbor,mi 48109-1078, United States, angiogenesis research laboratory,department of restorative sciences,university of michigan school of dentistry,1011 n. university,ann arbor,mi 48109-1078, United States, center for craniofacial molecular biology,school of dentistry,university of southern california,los angeles,ca, United States, department of pediatric dentistry,school of dentistry,federal university of rio grande do sul, Brazil, angiogenesis research laboratory,department of restorative sciences,university of michigan school of dentistry,1011 n. university,ann arbor,mi 48109-1078,united states,department of otolaryngology,university of michigan school of medicine,united states,department of biomedical engineering,university of michigan college of engineering, United States
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Authors
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