اثر خوراک حاوی پروبیوتیک lactobacillus acidophilus و پلی‌فنول فرولیک اسید بر ایمنی غیراختصاصی و فعالیت آنزیم‌‌های آنتی‌اکسیدانی در ماهی قرمز (carassius auratus)

در مطالعه حاضر، تاثیر جیره‌‌های حاوی پروبیوتیکlactobacillus acidophilus   (la) و پلی‌‌فنول فرولیک اسید (fa) را بر عملکرد رشد، میزان ایمنی غیر اختصاصی، و فعالیت آنزیم‌‌های آنتی‌‌اکسیدانی در ماهی قرمز (carassius auratus) مورد بررسی قرار گرفت. 240 عدد ماهی با وزن اولیه 0.35±3.34 گرم با چهار جیره آزمایشی شامل مقدار صفر از مکمل‌‌های غذایی (t0)، 108× 6 واحد تشکیل دهنده‌‌ کلنی بر گرم پروبیوتیک la (t1)، 100 میلی‌گرم بر کیلوگرم fa (t2) و ترکیبی از la و fa (t3) به مدت 8 هفته تغذیه شدند. نتایج نشان داد که وزن نهایی، افزایش وزن و نرخ رشد ویژه به طور قابل‌توجهی تحت تاثیر la و fa قرار گرفتند و بیشترین رشد در تیمار t3 مشاهده شد (p<0.05). سطوح ایمونوگلوبولین تام، igm و لیزوزیم با رژیم غذایی fa و la افزایش یافت (0.05>p). سطوح ایمونوگلوبولین تام، igm و لیزوزیم با رژیم غذایی fa و la افزایش یافت. فعالیت مالون دی آلدئید (mda) به‌وسیله جیره‌های آزمایشی کاهش یافت و کمترین مقدار آن به طور قابل‌توجهی در تیمار t3 در مقایسه با گروه شاهد مشاهده شد (0.05>p). ماهی‌هایی که با جیره‌های حاوی la یا fa تغذیه شده بودند، افزایش قابل‌توجهی در فعالیت کاتالاز (cat)، سوپراکسید دیسموتاز (sod) و گلوتاتیون پراکسیداز (gpx) نسبت به گروه شاهد نشان دادند (0.05>p). نتایج نشان داد که این مکمل‌‌های غذایی به صورت هم‌زمان در غلظت‌‌های 100 میلی‌‌گرم بر کیلو‌‌گرم فرولیک اسید و 108× 6 کلنی بر گرم پروبیوتیک la (t3)، از پتانسیل افزایش رشد، ایمنی ذاتی و آنزیم‌‌های آنتی‌‌اکسیدانی در ماهی قرمز برخوردارند. هر دو مکمل خوراکی اثرات هم‌افزایی نشان دادند و توصیه می‌‌شود از این ترکیبات به طور هم‌زمان برای آبزی‌پروری پایدار استفاده شود.

effect of dietary probiotic lactobacillus acidophilus and polyphenol ferulic acid on non-specific immunity and antioxidant activity in goldfish (carassius auratus)

introductionfish is among the best sources of animal protein but aquaculture industry have faced to many challenges. global aquaculture productions are vulnerable, and the increasing prevalence of diseases has caused a partial and overall reduction in aquaculture production (bondad-reantaso et al., 2005). factors such as overcrowding in ponds, periodic movement and manipulation, sudden temperature changes, water quality deterioration, and poor nutritional conditions, along with physiological changes in fish such as stress, have heightened susceptibility to infections (quesada-garcía et al., 2013). in aquaculture, the application of natural immuno-stimulants to enhance fish health and boost their resistance to pathogens has shown encouraging results (habibnia et al., 2024).methodologythis study examined the impact of incorporating probiotic lactobacillus acidophilus (la) and polyphenol ferulic acid (fa) into the diet of goldfish (carassius auratus) on growth performance, as well as factors related to immunity and antioxidant activity. a total of 240 fish, initially weighing 3.34 ± 0.35 g, were divided into four experimental groups: a control treatment with no food supplement (t0), fed with 6 x 108 cfu/g la probiotic (t1), fed with 100 mg/kg fa (t2), and fed with a combination of la and fa (t3) for eight weeks. at the end of feeding trial, the fish were fasted for 24 hours and all the fish in each tank were sampled and then anesthetized with benzocaine at a concentration of 120 mg per liter and weighed individually. growth performance was then assessed. thereafter, nine fish (per replication) were randomly selected. the selected fish were then subjected to anesthesia with benzocaine at a concentration of 120 mg per liter to minimize handling stress. the fish underwent dissection, with the intestine and liver tissues being meticulously separated. subsequently, these tissues were promptly subjected to freezing using liquid nitrogen and were subsequently preserved at a temperature of -80 °c. to measure lysozyme activity, the sample was added to a suspension of micrococcus lysodeikticus prepared in 0.1 molar citrate phosphate buffer at a ph of 5.8. optical density was read at a wavelength of 410 nanometers for 5 minutes, with readings taken every 30 seconds. total immunoglobulin (ig) levels were determined based on the method by siwicki et al. (1994). in brief, total protein from the homogenate sample was measured using the microprotein method, followed by precipitation of immunoglobulin molecules with a 12% polyethylene glycol solution. the protein level was then re-measured, and the difference in protein content was considered as the ig content. the levels of lipid peroxidation products in the fish homogenate were determined based on a previous study (kei, 1978). briefly, trichloroacetic acid (20%) (1.25 ml) was mixed with the fish homogenate (0.25 ml) and centrifuged at 2000 g for 10 minutes. then, 1.25 ml of sulfuric acid (0.05 molar) and 1 ml of thiobarbituric acid (0.2%) were added to the collected precipitate and boiled for 30 minutes. after adding 2 ml of n-butanol, centrifugation (2000 g) was performed for another 10 minutes. the final absorbance was recorded at a wavelength of 532 nanometers. catalase activity in the fish homogenate was assessed based on a previous study (goth, 1991). the reaction buffer (1 ml) consisted of hydrogen peroxide (65 mm) and sodium-potassium phosphate buffer (60 mm) mixed with the fish homogenate (0.5 ml) for 1 minute at 37°c. the enzymatic reaction was terminated by adding 1 ml of ammonium molybdate (32.4 mm), and absorbance was recorded at 405 nanometers. superoxide dismutase activity in the fish homogenate was determined based on a previous study (nishiimi et al., 1997). the reaction buffer included 2.6 ml of phosphate buffer (0.017 mm), 0.1 ml of phenazine methosulfate, and 0.1 ml of nitro blue tetrazolium mixed with the fish homogenate (0.5 ml).