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Genetic diversity of Penicillium species isolated from various sources in Sarawak,Malaysia [Malezya sarawak'ta farkli{dotless} kaynaklardan elde edilen Penicillium türlerinin genetik çeşitliliǧi]
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نویسنده
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roslan h.a. ,ngo c.s. ,muid s.
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منبع
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journal of cell and molecular biology - 2010 - دوره : 7-8 - شماره : 2-1 - صفحه:13 -23
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چکیده
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Borneo island is one of the megadiversity centres of the world and contain vast amount of flora and fauna. the penicillium species are among the most commonly occurring and economically important members of micro-fungi family. in this study,morphological and random amplification polymorphic dna (rapd) molecular methods were used to group and determine genetic variability and relationship among twenty penicillium isolates from various locations in western part of borneo island that was maintained in the pure culture collection of university malaysia sarawak. comparison between morphological and molecular method using m13 and opd10 primers were undertaken and showed that in some cases,the groupings of isolates based on morphological method were consistent with molecular groupings with a few exceptions. molecular analysis also indicated genotype variability between the isolates with little correlation with either the origin of soil or geographical location.
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کلیدواژه
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M13; Morphology; Penicillium species; RAPD; Variation
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آدرس
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department of molecular biology,faculty of resource science and technology,universiti malaysia sarawak,94300 kota samarahan, Malaysia, department of molecular biology,faculty of resource science and technology,universiti malaysia sarawak,94300 kota samarahan, Malaysia, department of plant sciences and environmental ecology,faculty of resource science and technology,universiti malaysia sarawak,94300 kota samarahan, Malaysia
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Authors
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