Eukaryotic elongation factor eEF1A1 promotes and Ser300 mutants of eEF1A1 inhibit transition through the S and G2/M phases of the cell cycle [Ökaryotik uzama faktörü eEF1A1 G2/M geçişini destekler ve eEF1A1S'nin Ser300 mutanti{dotless} engeller]

The eukaryotic elongation factor 1 alpha (eef1a) plays a crucial role in normal physiological processes and carcinogenic transformation. our recent study showed that the phosphorylation of eef1a1 at ser300 by type i transforming growth factor-β (tgf-β) receptor inhibited the protein synthesis and cell proliferation. inhibition of cell proliferation may occur by accumulation of cells in a specific phase of the cell cycle. we report here an exploration of the role of eef1a1 and an impact of ser300 in eef1a1 on the transition through the phases of the cell cycle by human breast epithelial cells. our results suggest that the overexpression of eef1a1 increases proliferation of cells by promoting transition of cells through the s- and g2/m-phases in the cell cycle. ser300 mutants of eef1a1 with impaired ability to support protein synthesis inhibited the accumulation of cells in g0/g1 phase. thus,we showed that eef1a1 modulates transition of the s and g2/m phases by cells.