Growth inhibitory effects of a GlcNAc/GalNAc-specific lectin from the marine demosponge Halichondria okadai on human pathogenic microorganisms [Denizel Demospongia Halichondira okadai GlcNac/GalNAc-özgün lektininin insan patojenik mikroorganizmalari{dotless} üzerindeki büyüme önleyici etkileri]

The aim of this study was to demonstrate the microbial usefulness of glcnac/galnac-specific lectin obtained from the marine demosponge,halichondria okadai by affinity chromatography. for this purpose,we investigated the growth inhibitory effects of this lectin on bacterial strains. through sds-polyacrylamide gel electrophoresis,lectin was found to be a 18 kda polypeptide. this lectin showed significant hemagglutinating activity against trypsinized human and rabbit erythrocytes and it was inhibited by n-acetyl glucosamine (glcnac) and n-acetyl galactosamine (galnac). lectin was screened for in vitro antibacterial activity against eleven human pathogenic bacteria and significantly inhibited the growth of gram-positive bacteria more than gram-negative bacteria. antibacterial evaluation of standard antibodies,ampicillin was used as comparative study. bacillus megaterium (13±1 mm) and staphylococcus aureus (11±1 mm) were exhibited the highest zone of inhibition by the addition of the lectin (250 μg/disc). at the same time,lectin showed good growth inhibition against the gram-negative bacteria such as shigella sonnei (10±1 mm),salmonella typhi (08±1 mm) and vibrio cholerae (07±1 mm). however,it did not inhibit the growth of gram-negative bacteria as escherichia coli and pseudomonas sp. these antibacterial results indicate that future findings of lectin applications obtained from marine demosponge may be of importance to clinical microbiology and could have possible applications in therapeutic.