In vitro regeneration of Cleome viscosa - an important medicinal herb [Önemli bir ti{dotless}bbi bitki olan Cleome viscosa'ni{dotless}n in vitro rejenerasyonu]

Cleome viscosa is an important medicinal herb. mass collection of the plant from natural habitats has led to its depletion. we have established a protocol for its mass propagation through in vitro organogenesis using leaf explants. for callus induction it was found that indole-3acetic acid (2mg/l) has a significant effect on the callus induction,callus index and callus physical appearance. the moderate response was observed with indole-3butyric acid (2mg/l),and a low response was observed with 2-napthalene acetic acid (2mg/l). 2mg/l concentration of benzyl aminopurine significantly influenced shoot proliferation. optimum response was observed with benzyl aminopurine + kinetin (5+2.5mg/l),and low response was observed with kinetin (1mg/l). about 91% of rooting response was observed with naa (0.1mg/l). moderate response was observed from indole-3butyric acid (0.1mg/l) and low response observed from iaa (0.1mg/l). after two weeks of acclimatization 90% of survival rate have been observed. the present study describes a well-documented and reliable protocol for cleome viscosa from leaf explants with a high rate of multiplication. this protocol could be used as a basic tool for commercial cultivation of this medicinally important plant cleome viscosa.