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Identification of silencing suppressors of potato virus M [Patates virüsü M'nin susturucu baski{dotless}layi{dotless}ci{dotless}lari{dotless}ni{dotless}n tayini]
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نویسنده
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kryldakov r. ,akbergenov r. ,hohn t. ,iskakov b.
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منبع
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journal of cell and molecular biology - 2011 - دوره : 9 - شماره : 1 - صفحه:15 -20
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چکیده
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Gene silencing is an important regulatory and defense mechanism in plants. plant viruses evolved some proteins that suppress cellular rna interference mechanism. in this work identification of silencing suppressors of potato virus m (pvm) is reported. the cdna sequences of different pvm open reading frames were cloned into the gateway-compatible binary t-dna destination vector pmdc32,harboring a double 35s promoter. each of these constructs was electroporated into agrobacterium tumefaciens c58c1 which then were agroinfiltrated into nicotiana benthamiana 16c line. it was shown that viral coat protein (34k) as well as some other pvm proteins (12k,11k) possessed suppressor properties of cellular silencing activity.
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کلیدواژه
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Agroinfiltration; Gene silencing; Nicotiana benthamiana 16C line; Potato virus M; Suppressors of silencing
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آدرس
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laboratory of proteins and nucleic acids research,m.a. ajtkhozhin institute of molecular biology and biochemistry, Kazakhstan, laboratory of molecular plant virology,botanical institute,university of basel, Switzerland, laboratory of molecular plant virology,botanical institute,university of basel, Switzerland, laboratory of proteins and nucleic acids research,m.a. ajtkhozhin institute of molecular biology and biochemistry, Kazakhstan
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Authors
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