HIV-1 reverse transcriptase inhibition by Vitex negundo L. leaf extract and quantification of flavonoids in relation to anti-HIV activity [Vitex negundo L. yaprak özütüyle HIV-1 ters transkriptaz inhibisyonu ve anti- HIV aktivitesiyle ilişkili flavonoidlerin kantifikasyonu üzerine bir çali{dotless}şma]

This study aimed to determine the activity of ethanolic leaf extract of vitex negundo l. against hiv-1 reverse transcriptase (rt) and to identify and quantify the flavonoids present. the effects of ethanolic (85%) leaf extract of vitex negundo l. on rt activity in vitro were evaluated with recombinant hiv-1 enzyme,using a non-radioactive hiv-rt colorimetric elisa kit. in addition,identification and quantification of flavonoids such as rutin,luteolin,myricetin,quercetin,kaempherol,isorhamnetin and quercetagetin were analysed using hplc. the plant vitex negundo l. ethanolic leaf extract exhibited the most notable activity of 92.8% against hiv-1 rt at 200 μg/ml concentration. phytochemical analysis revealed the presence of steroids,triterpenes,alkaloids,flavanoids,antroquinone glycosides and amino acids. among 7 flavonoids tested,6 were identified in the decreasing order of quantity as kaempherol,myricetin,quercetin,quercetagetin,isorhamnetin and luteolin. the study revealed that the plant vitex negundo l. leaf possess anti-rt substances and probably the flavonoids act as anti-virus agents.