Study of Klebsiella pneumoniae isolates with ESBL activity,from ICU and Nurseries,on the island of Mauritius [Mauritius adasi{dotless}nda kreş ve yoǧun baki{dotless}m ünitesinden elde edilen ESBL aktiviteli Klebsiella pneumoniae izolatlari{dotless}ni{dotless}n çali{dotless}şmasi{dotless}]

Klebsiella pneumoniae with extended spectrum beta lactamase activity circulate widely among nosocomial environments. isolates were collected from hospitalised patients in icu and nursery units on the island of mauritius,over a two year period. they were tested for their resistance/susceptibility to various antibiotics using the double disc diffusion assay. following the api biochemical assay their genus/species were confirmed. their genetic diversity was determined using rapd,rep and boxpcr. among fifty isolates,most were highly diverse by these methods with a clustering into three groups. no correlation was found among isolates of the same hospital or unit or with dates of collection. sequence analysis of parts of the tem,shv and ctx-m beta lactamase genes confirmed the beta lactamase domain and the presence of shv-11 and shv-28. ctx-m 15 was found in some isolates. furthermore,integrase was pcr-amplified from a few isolates,showing the presence of an integron-borne gene cassette. this study shows diverse clones of klebsiella pneumoniae circulating with esbl activity.