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Study of Klebsiella pneumoniae isolates with ESBL activity,from ICU and Nurseries,on the island of Mauritius [Mauritius adasi{dotless}nda kreş ve yoǧun baki{dotless}m ünitesinden elde edilen ESBL aktiviteli Klebsiella pneumoniae izolatlari{dotless}ni{dotless}n çali{dotless}şmasi{dotless}]
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نویسنده
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mungloo-rujubali s.k. ,issack m.i. ,jaufeerally-fakim y.
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منبع
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journal of cell and molecular biology - 2012 - دوره : 10 - شماره : 2 - صفحه:39 -51
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چکیده
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Klebsiella pneumoniae with extended spectrum beta lactamase activity circulate widely among nosocomial environments. isolates were collected from hospitalised patients in icu and nursery units on the island of mauritius,over a two year period. they were tested for their resistance/susceptibility to various antibiotics using the double disc diffusion assay. following the api biochemical assay their genus/species were confirmed. their genetic diversity was determined using rapd,rep and boxpcr. among fifty isolates,most were highly diverse by these methods with a clustering into three groups. no correlation was found among isolates of the same hospital or unit or with dates of collection. sequence analysis of parts of the tem,shv and ctx-m beta lactamase genes confirmed the beta lactamase domain and the presence of shv-11 and shv-28. ctx-m 15 was found in some isolates. furthermore,integrase was pcr-amplified from a few isolates,showing the presence of an integron-borne gene cassette. this study shows diverse clones of klebsiella pneumoniae circulating with esbl activity.
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کلیدواژه
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Beta lactamase; ESBL; Genetic diversity; Klebsiella pneumoniae; SHV; TEM
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آدرس
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department of biotechnology,university of mauritius, Mauritius, victoria hospital, Mauritius, department of biotechnology,university of mauritius, Mauritius
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Authors
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