Cellular transdifferentiation into brown adipose-like cells by adenoviral-directed expression or stable transfection of HB-EGF and ADAM 12s [HB-EGF ve ADAM 12S'in adenovirüsle yönlendirilmiş ifadesi veya kal?c? Transfeksiyonu yoluyla kahverengi adipoz benzeri hücrelere hücresel transdiferansiyasyon]

Adenoviral vectors directing the expression of hb-egf and adam 12s were used to better understand the gene expression profile of cellular reprogramming into bat-like cells compared to hb-egf and adam 12s transfection. human epidermoid carcinoma cells (a431) were co-infected with hb-egf and adam 12s adenoviruses,monitored for fluorescence and lipid accumulation for 1-3 weeks. cells were stained with oil red o and total rna was harvested and analyzed with a human adipogenic rt2 profiler array. additionally,the gene expression profile of rna from a431 control cells and a431 cells stably transfected with hb-egf and adam 12s were analyzed and compared to the gene expression patterns of adenoviral hb-egf and adam 12s infected cells. adenoviral hb-egf adam 12s coinfected a431 rna exhibited significant up-regulation of pgc-1α,klf3,klf4 and fgf-2 mrna and downregulation of c/ebpα,lmna,glut4 and src mrna. rna derived from hb-egf and adam 12s co-transfected a431 cells exhibited similar gene expression profiles with the human adipogenic array. adenovirus hb-egf and adam 12s co-infected cells transdifferentiate human a431 cells into a bat-like phenotype and recapitulate findings with hb-egf and adam 12s transfected cells. the findings presented in this study identify genes important in bat-like cellular reprogramming and may have therapeutic implications to obesity and type 2 diabetes. © 2014 journal of cell and molecular biology. all rights reserved.