Covalent immobilization of lysozyme onto stainless steel as an anti-biofilm coating

Covalent immobilization of an enzyme for use as antimicrobial biocompatible surface applications was performed utilizing the sol-gel chemistry,illustrated by chicken egg white lysozyme. the sol-gel network,which contains amine functional groups,provides covalent attachment sites for the enzyme via the carbodiimide reaction. as an alternative method,these free amine groups of the sol-gel layer were utilized for the immobilization of lysozyme from its n-terminus by using poly(acrylic acid) brushes as the linker molecule. the enzymatic activity of covalently immobilized lysozyme with both of the procedures has been investigated. © 2014 journal of cell and molecular biology. all rights reserved.