Regional contrast agent quantification in a mouse model of myocardial infarction using 3D cardiac T 1mapping

Background: quantitative relaxation time measurements by cardiovascular magnetic resonance (cmr) are of paramount importance in contrast-enhanced studies of experimental myocardial infarction. first,compared to qualitative measurements based on signal intensity changes,they are less sensitive to specific parameter choices,thereby allowing for better comparison between different studies or during longitudinal studies. secondly,t 1measurements may allow for quantification of local contrast agent concentrations. in this study,a recently developed 3d t 1mapping technique was applied in a mouse model of myocardial infarction to measure differences in myocardial t 1before and after injection of a liposomal contrast agent. this was then used to assess the concentration of accumulated contrast agent. materials and methods. myocardial ischemia/reperfusion injury was induced in 8 mice by transient ligation of the lad coronary artery. baseline quantitative t 1maps were made at day 1 after surgery,followed by injection of a gd-based liposomal contrast agent. five mice served as control group,which followed the same protocol without initial surgery. twenty-four hours post-injection,a second t 1measurement was performed. local r 1values were compared with regional wall thickening determined by functional cine cmr and correlated to ex vivo gd concentrations determined by icp-ms. results: compared to control values,pre-contrast t 1of infarcted myocardium was slightly elevated,whereas t 1of remote myocardium did not significantly differ. twenty-four hours post-contrast injection,high r 1values were found in regions with low wall thickening values. however,compared to remote tissue (wall thickening > 45%),r 1was only significantly higher in severe infarcted tissue (wall thickening < 15%). a substantial correlation (r = 0.81) was found between cmr-based r 1values and gd concentrations from ex vivo icp-ms measurements. furthermore,regression analysis revealed that the effective relaxivity of the liposomal contrast agent was only about half the value determined in vitro. conclusions: 3d cardiac t 1mapping by cmr can be used to monitor the accumulation of contrast agents in contrast-enhanced studies of murine myocardial infarction. the contrast agent relaxivity was decreased under in vivo conditions compared to in vitro measurements,which needs consideration when quantifying local contrast agent concentrations. © 2011 coolen et al; licensee biomed central ltd.