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Hyperemic stress myocardial perfusion cardiovascular magnetic resonance in mice at 3 Tesla: Initial experience and validation against microspheres
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نویسنده
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jogiya r. ,makowski m. ,phinikaridou a. ,patel a.s. ,jansen c. ,zarinabad n. ,chiribiri a. ,botnar r. ,nagel e. ,kozerke s. ,plein s.
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منبع
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journal of cardiovascular magnetic resonance - 2013 - دوره : 15 - شماره : 1
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چکیده
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Background: dynamic first pass contrast-enhanced myocardial perfusion is the standard cmr method for the estimation of myocardial blood flow (mbf) and mbf reserve in man,but it is challenging in rodents because of the high temporal and spatial resolution requirements. hyperemic first pass myocardial perfusion cmr during vasodilator stress in mice has not been reported. methods. five c57bl/6 j mice were scanned on a clinical 3.0 tesla achieva system (philips healthcare,netherlands). vasodilator stress was induced via a tail vein catheter with an injection of dipyridamole. dynamic contrast-enhanced perfusion imaging (gadobutrol 0.1 mmol/kg) was based on a saturation recovery spoiled gradient echo method with 10-fold k-space and time domain undersampling (k-t pca). one week later the mice underwent repeat anaesthesia and lv injections of fluorescent microspheres at rest and at stress. microspheres were analysed using confocal microscopy and fluorescence-activated cell sorting. results: mean mbf at rest measured by fermi-function constrained deconvolution was 4.1 ± 0.5 ml/g/min and increased to 9.6 ± 2.5 ml/g/min during dipyridamole stress (p = 0.005). the myocardial perfusion reserve was 2.4 ± 0.54. the mean count ratio of stress to rest microspheres was 2.4 ± 0.51 using confocal microscopy and 2.6 ± 0.46 using fluorescence. there was good agreement between cardiovascular magnetic resonance cmr and microspheres with no significant difference (p = 0.84). conclusion: first-pass myocardial stress perfusion cmr in a mouse model is feasible at 3 tesla. rest and stress mbf values were consistent with existing literature and perfusion reserve correlated closely to microsphere analysis. data were acquired on a 3 tesla scanner using an approach similar to clinical acquisition protocols,potentially facilitating translation of imaging findings between rodent and human studies. © 2013 jogiya et al.; licensee biomed central ltd.
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کلیدواژه
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Cardiovascular magnetic resonance imaging; Murine; Myocardial perfusion
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آدرس
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division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, academic department of surgery,bhf centre of excellence,kings college,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london, United Kingdom, division of imaging sciences,epsrc medical engineering centre,rayne institute,london,united kingdom,institute for biomedical engineering,university and eth zurich,zurich, Switzerland, division of imaging sciences,epsrc medical engineering centre,rayne institute,london,united kingdom,multidisciplinary cardiovascular research centre,leeds institute of genetics,health and therapeutics,university of leeds,leeds,ls2 9jt, United Kingdom
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Authors
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