Association of the lox-1 rs1050283 polymorphism with risk for atherosclerotic cerebral infarction and its effect on slox-1 and lox-1 expression in a chinese population

Aims: the interaction between lectin-like oxidized low density lipoprotein (ldl) receptor-1 (lox-1) and oxidized ldl (ox-ldl) has been viewed as an important pathogenic factor for cardiovascular diseases. this study aimed to explore the association of a functional polymorphism rs1050283 in the 3′-untranslated region of the lox-1 gene with atherosclerotic cerebral infarction (aci) susceptibility,and we also investigated the effects of the rs1050283 polymorphism on lox-1 expression and serum levels of slox-1 in patients with aci. methods: a case-controlled study was performed in 526 patients with aci and 640 healthy controls. genotyping was performed by dna sequencing method. real-time pcr and western blotting were used to determine the level of lox-1 expression. serum levels of slox-1 were quantified using elisa according to the manufacturer’s instruction. results: the results of the present study showed that the frequency of rs1050283 t allele was significantly higher in patients with aci than in healthy controls. we also found that the rs1050283 polymorphism t allele was associated with increased lox-1 expression at mrna and protein levels in patients with aci. furthermore,we also observed that among patients with aci,those with the rs1050283 t allele showed an increased serum level of slox-1. conclusion: our research demonstrated that the rs1050283 t allele of lox-1 is strongly associated with an increased risk for aci in a chinese population,which also affects levels of lox-1 and slox-1. © 2017 japan atherosclerosis society.