|
|
|
|
A promoter in the novel exon of hPPARγ directs the circadian expression of PPARγ
|
|
|
|
|
|
|
|
نویسنده
|
takahashi s. ,inoue i. ,nakajima y. ,seo m. ,nakano t. ,yang f. ,kumagai m. ,komoda t. ,awata t. ,ikeda m. ,katayama s.
|
|
منبع
|
journal of atherosclerosis and thrombosis - 2010 - دوره : 17 - شماره : 1 - صفحه:73 -83
|
|
چکیده
|
Aim: pparγ (peroxisome proliferator-activated receptor γ) is a member of the nuclear receptor superfamily of ligand-activated transcription factors that regulate the expression of genes associated with lipid metabolism. herein,we show that expression levels of the novel pparγ transcript exhibit circadian oscillation. to study the mechanisms controlling pparγ expression,a novel pparγ gene promoter was cloned and characterized. methods: we analyzed the novel pparγ promoter by luciferase reporter assays and gel shift analysis. results: surprisingly,it was not an intron but rather the novel first exon of pparγ that was found to have functional minimal promoter activity. luciferase reporter assays and gel shift assays revealed that the novel first exon is essential for novel pparγ promoter activation and that dbp (albumin gene d-site binding protein) and e4bp4 (e4 promoter a binding protein 4) bind directly to d-sites in the novel first exon. conclusion: our results demonstrate that the par-bzip (bzip,basic leucine zipper) family and e4bp4 are the main regulatory factors involved in oscillation of novel pparγ expression. this regulatory mechanism clearly differs from that of the circadian expression of pparγ.
|
|
کلیدواژه
|
DBP; E4BP4; PAR-bZIP; PPARγ
|
|
آدرس
|
department of diabetes and endocrinology,saitama medical university,38 morohongo,moroyama,iruma-gun,saitama 350-0495,japan,department of biochemistry,saitama medical university,saitama, Japan, department of diabetes and endocrinology,saitama medical university,38 morohongo,moroyama,iruma-gun,saitama 350-0495, Japan, cell dynamics research group,research institute for cell engineering,national institute of advanced industrial science and technology (aist),osaka, Japan, department of biochemistry,saitama medical university,saitama, Japan, department of biochemistry,saitama medical university,saitama, Japan, department of physiology,saitama medical university,saitama,japan,molecular clock project,research center for genomic medicine,saitama medical university,saitama, Japan, department of physiology,saitama medical university,saitama,japan,molecular clock project,research center for genomic medicine,saitama medical university,saitama, Japan, department of biochemistry,saitama medical university,saitama, Japan, department of diabetes and endocrinology,saitama medical university,38 morohongo,moroyama,iruma-gun,saitama 350-0495, Japan, department of physiology,saitama medical university,saitama,japan,molecular clock project,research center for genomic medicine,saitama medical university,saitama, Japan, department of diabetes and endocrinology,saitama medical university,38 morohongo,moroyama,iruma-gun,saitama 350-0495, Japan
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|