Bisphenol a downregulates akt signaling and inhibits adiponectin production and secretion in 3T3-L1 adipocytes

Aim: the aim of this study was to investigate whether environmental endocrine-disrupting chemicals,bisphenol a (bpa) and bpa-related chemicals,affect adiponectin production and secretion in 3t3-l1 adipocytes and whether bpa acts through akt signaling. & methods: 3t3-l1 adipocytes were treated for 24 h with bpa at various concentrations (20-80 μm) in serum-deprived medium. the medium was filtered through a 0.2 μm filter. adiponectin in the infranatants of cell homogenates and in the media was measured using an adiponectin elisa kit. the levels of akt and p-akt in cultures treated for 24 h with or without 80 μm bpa were analyzed by western blot. &results: the control cultures (i.e.,bpa was absent during a 24-h treatment period) contained 49.4 μg/mg dna of adiponectin in the cells and secreted 35.5 μg/mg dna of adiponectin into the medium. bpa at 80 μm dose-dependently decreased the amounts of intracellular and medium adiponectin by 60% (p<0.01) and 56% (p<0.01),respectively,and decreased the levels of akt and p-akt by 46% (p <0.01) and 29% (p<0.01),respectively,compared with the control cultures. like bpa,bisphenol f (bpf),bisphenol e (bpe),and bisphenol b (bpb) decreased the amounts of intracellular and medium adiponectin. the order of the potential to decrease the amount of intracellular adiponectin was bpb>bpa>bpe>bpf. & conclusions: bpa downregulates akt signaling and inhibits adiponectin production and secretion in 3t3-l1 adipocytes.