Carriage of the V279F homozygous genotype,a rare allele,within the gene encoding Lp-PLA2 leads to changes in circulating intermediate metabolites in individuals without metabolic syndrome

Aim: identifying differences in plasma metabolic profiling between lp-pla2 279vv and 279ff in individuals without metabolic syndrome (mets) can be used to elucidate the roles of novel lp-pla2 activities in normal physiological processes.methods: non-mets individuals with 279ff (n = 36) and age-,sex- and bmi-matched vv subjects (n =36) were included in this analysis.results: the ff subjects exhibited no appreciable enzyme activity. no significant differences were observed between the vv and ff subjects in the serum lipid profiles or hs-crp,plasma ox-ldl,mda or urinary 8-epi-pgf2 α levels. the ff subjects also showed lower activities of lyso-phosphatidylcholine (lysopc) (16:0) (p=0.003) and oleamide (p＜0.001) and a higher activity of l-tryptophan (p=0.016) than the vv subjects. in addition,the lp-pla2 activity positively correlated with the lysopc (16:0) and lysopc (18:0) activities and negatively correlated with the pc (16:0/22:6) and l-tryptophan activities in the vv subjects. furthermore,in the vv subjects,the lysopc (16:0) and lysopc (18:0) activities negatively correlated with the presence of pcs containing 14:0/20:2,14:0/22:4 and 16:0/22:6,while the oleamide activity exhibited a strong positive correlation with lysopcs and a negative correlation with pcs,whereas the relationship between oleamide and lysopcs and pcs was weaker in the ff subjects.conclusions: the present results indicate that the natural absence of the plasma lp-pla2 activity due to carriage of the lp-pla2 279ff genotype may reduce the generation of lysopc (16:0) and oleamide and thereby enhance the activity of plasma tryptophan in normal physiological processes. © 2014,japan atherosclerosis society. all rights reserved.