Effect of taurine chloramine on the production of matrix metalloproteinases (MMPs) in adiponectin- or IL-1-stimulated fibroblast-like synoviocytes

Background. adiponectin greatly stimulated the expression of matrix metalloproteinases (mmps) in fibroblast-like synoviocytes (flss) as did il-1. we wondered whether taurine chloramine (taucl) inhibits the production of mmps stimulated by adiponectin in the same pattern as by il-1 stimulation in vitro. methods. synovial cells from rheumatoid arthritis (ra) patients were treated with adiponectin or interleukin (il)-1 for 24 hr in the presence or absence of taucl. the culture supernatant was collected and the levels of mmps were measured by enzyme-linked immunosorbent assay (elisa). the ib signaling pathways stimulated by adiponectin were studied and the levels of nf-b in the nuclei of the cells were analyzed by elisa. results. taucl (600 νm) inhibited mmp-13,but not mmp-1,expression in il-1-stimulated ra flss. however,taucl at the same concentration significantly inhibited the production of both adiponectin-stimulated mmp-1 and mmp-13 expression. taucl inhibited the degradation of ib- stimulated by adiponectin,but not by il-1. similarly,the level of nf-b in the nucleus was increased by adiponectin stimulation and was inhibited by 600 νm taucl. however,the levels of nf-b increased by il-1 stimulation were not inhibited by 600 νm taucl. conclusions. taucl more effectively inhibited mmps expression induced by adiponectin than that by il-1 in ra fls,suggesting that taucl plays an important role in down-regulating the expression of mmps in arthritic joints. © 2010 kim et al; licensee biomed central ltd.