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Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells
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نویسنده
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lin c.-r. ,chen k.-h. ,yang c.-h. ,cheng j.-t. ,sheen-chen s.-m. ,wu c.-h. ,sy w.-d. ,chen y.-s.
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منبع
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journal of biomedical science - 2010 - دوره : 17 - شماره : 1
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چکیده
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Background: gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. however,effective transfection of postmitotic neurons remains a challenge. the aim of this study was to develop a method for gene transfer into rat primary dorsal root ganglion neurons using sonoporation. methods: dissociated cells from adult rat dorsal root ganglion (drg) cells were sonicated for 1-8 s at 2.5-10 w to determine the optimal ultrasound duration and power for gene transfection and cell survival. transfection efficiency was compared between sonoporation,liposome and lentiviral vector gene transfer techniques. results: the optimum ultrasound intensity was 5 w for 2 s and yielded an efficiency of gene transfection of 31% and a survival rate of 35%. conclusions: sonoporation can be optimized to minimize cell death and yield a high percentage of transfected neurons and that this technique can be easily applied to primary cultures of rat dorsal root ganglion neurons. © 2010 lin et al; licensee biomed central ltd. t.
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آدرس
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department of anesthesiology,chang gung memorial hospital-kaohsiung,medical center,chang gung university,college of medicine,kaohsiung,taiwan,department of anesthesiology,national taiwan university,college of medicine,taipei, Taiwan, department of anesthesiology,chang gung memorial hospital-kaohsiung,medical center,chang gung university,college of medicine,kaohsiung, Taiwan, department of anesthesiology,chang gung memorial hospital-kaohsiung,medical center,chang gung university,college of medicine,kaohsiung, Taiwan, department of biological sciences,national sun yat-sen university,kaohsiung, Taiwan, department of surgery,chang gung memorial hospital-kaohsiung,medical center,chang gung university,college of medicine,kaohsiung, Taiwan, department of anesthesiology,chang gung memorial hospital-kaohsiung,medical center,chang gung university,college of medicine,kaohsiung,taiwan,department of biological sciences,national sun yat-sen university,kaohsiung, Taiwan, department of anesthesiology,chang gung memorial hospital-kaohsiung,medical center,chang gung university,college of medicine,kaohsiung, Taiwan, department of anesthesiology,chang gung memorial hospital-kaohsiung,medical center,chang gung university,college of medicine,kaohsiung, Taiwan
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Authors
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