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In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles
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نویسنده
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chou m.-i. ,hsieh y.-f. ,wang m. ,chang j.t. ,chang d. ,zouali m. ,tsay g.j.
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منبع
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journal of biomedical science - 2010 - دوره : 17 - شماره : 1
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چکیده
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Background. rna interference (rnai) is a powerful tool to silence gene expression post-transcriptionally. delivering sequences of rnai in vivo remains a problem. the aim of this study was to use jc virus (jcv) virus-like particles (vlps) as a vector for delivering rnai in silencing the cytokine gene of il-10. methods. jcv vlps were generated by recombinant jcv vp1 protein in yeast expression system. dna fragment containing il-10 shrna was packaged into vlps by osmotic shock. results. in raw 264.7 cells,il-10 shrna was found to reduce il-10 expression by 85 to 89%,as compared with vlps alone. il-10 shrna did not cross-react with tnf-alpha mrna or influence the expression of tnf-alpha. in balb/c mice il-10 shrna could reduce 95% of il-10 secretion. surprisingly,it also down regulated tnf-alpha expression. conclusions. we show for the first time that jcv vlps empty capsids are competent vectors to deliver rnai and are nontoxic to cells,suggesting that jcv vlps is an efficient agent to deliver rnai in both murine macrophage cells and balb/c mice. this system provides an efficient means for delivering the rnai for gene therapy purposes. © 2010 chou et al; licensee biomed central ltd.
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آدرس
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institute of medicine,chung shan medical university,taichung, Taiwan, institue of immunology,chung shan medical university,taichung, Taiwan, department of microbiology and immunology,chung shan medical university,taichung, Taiwan, institute of medical and molecular toxicology,chung shan medical university,taichung,40201, Taiwan, department of life science,national chung cheng university,chiayi county, Taiwan, institute national de la santé et de la recherche médicale,inserm u606,hôpital lariboisière,2,paris cedex 10,france,university denis diderot paris 7,75475 paris, France, institute of medicine,chung shan medical university,taichung,taiwan,institue of immunology,chung shan medical university,taichung,taiwan,department of internal medicine,chung shan medical university hospital,taichung, Taiwan
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Authors
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