|
|
|
|
JNK suppression is essential for 17-Estradiol inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells
|
|
|
|
|
|
|
|
نویسنده
|
hsu h.-h. ,hu w.-s. ,lin y.-m. ,kuo w.-w. ,chen l.-m. ,chen w.-k. ,hwang j.-m. ,tsai f.-j. ,liu c.-j. ,huang c.-y.
|
|
منبع
|
journal of biomedical science - 2011 - دوره : 18 - شماره : 1
|
|
چکیده
|
Background: epidemiological studies demonstrate that the incidence and mortality rates of colorectal cancer in women are lower than in men. however,it is unknown if 17-estradiol treatment is sufficient to inhibit prostaglandin e2 (pge2)-induced cellular motility in human colon cancer cells. methods. we analyzed the protein expression of urokinase plasminogen activator (upa),tissue plasminogen activator (tpa),matrix metallopeptidases (mmps),plasminogen activator inhibitor-1 (pai-1) and tissue inhibitor of metalloproteinases (timps),and the cellular motility in pge2-stimulated human lovo cells. 17-estradiol and the inhibitors including ly294002 (akt activation inhibitor),u0126 (erk1/2 inhibitor),sb203580 (p38 mapk inhibitor),sp600125 (jnk1/2 inhibitor),qnz (nfb inhibitor) and ici 182 780 were further used to explore the inhibitory effects of 17-estradiol on pge2-induced lovo cell motility. student's t-test was used to analyze the difference between the two groups. results: upregulation of urokinase plasminogen activator (upa),tissue plasminogen activator (tpa) and matrix metallopeptidases (mmps) is reported to associate with the development of cancer cell mobility,metastasis,and subsequent malignant tumor. after administration of inhibitors including ly294002,u0126,sb203580,sp600125 or qnz,we found that pge2 treatment up-regulated upa and mmp-9 expression via jnk1/2 signaling pathway,thus promoting cellular motility in human lovo cancer cells. however,pge2 treatment showed no effects on regulating expression of tpa,mmp-2,plasminogen activator inhibitor-1 (pai-1),tissue inhibitor of metalloproteinase-1,-2,-3 and -4 (timp-1,-2,-3 and -4). we further observed that 17-estradiol treatment inhibited pge2-induced upa,mmp-9 and cellular motility by suppressing activation of jnk1/2 in human lovo cancer cells. conclusions: collectively,these results suggest that 17-estradiol treatment significantly inhibits pge2-induced motility of human lovo colon cancer cells. © 2011 hsu et al; licensee biomed central ltd.
|
|
|
|
|
آدرس
|
division of colorectal surgery,mackay memorial hospital,taipei,taiwan,mackay medicine,nursing and management college,taipei, Taiwan, division of cardiology,taipei medical university,shuang-ho hospital,taipei,taiwan,graduate institute of aging medicine,china medical university,taichung, Taiwan, school of medicine,chung shan medical university,taichung,taiwan,department of medical technology,jen-teh junior college of medicine,nursing and management,miaoli, Taiwan, department of biological science and technology,china medical university,taichung, Taiwan, division of medical technology,department of internal medicine,armed-force,taichung general hospital,taichung, Taiwan, emergency department,china medical university hospital,taichung, Taiwan, school of applied chemistry,chung shan medical university,taichung, Taiwan, emergency department,china medical university hospital,taichung, Taiwan, division of gastroenterology,department of internal medicine,kaohsiung medical university hospital,kaohsiung,taiwan,cancer center,kaohsiung medical university hospital,kaohsiung, Taiwan, department of pediatrics,medical research and medical genetics,china medical university,taichung,taiwan,graduate institute of basic medical science,china medical university,taichung,taiwan,department of health and nutrition biotechnology,asia university,taichung, Taiwan
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|