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Involvement of STIM1 and Orai1 in EGF-mediated cell growth in retinal pigment epithelial cells
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نویسنده
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yang i.-h. ,tsai y.-t. ,chiu s.-j. ,liu l.-t. ,lee h.-h. ,hou m.-f. ,hsu w.-l. ,chen b.-k. ,chang w.-c.
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منبع
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journal of biomedical science - 2013 - دوره : 20 - شماره : 1
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چکیده
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Background: in non-excitable cells,one major route for calcium entry is through store-operated calcium (soc) channels in the plasma membrane. these channels are activated by the emptying of intracellular ca§ssup§ 2+§esup§ store. stim1 and orai1 are major regulators of soc channels. in this study,we explored the functions of stim1 and orai1 in epidermal growth factor (egf)-induced cell proliferation and migration in retinal pigment epithelial cells (arpe-19 cell line). results: egf triggers cell proliferation and migration in arpe-19 cells. cell proliferation and migration involve stim1 and orai1,as well as phosphorylation of extracellular signal-regulated protein kinase (erk) 1/2,and akt. pharmacological inhibitors of soc channels and sirna of orai1 and stim1 suppress cell proliferation and migration. pre-treatment of mitogen-activated protein kinase kinase (mek) inhibitors and a phosphatidylinositol 3 kinases (pi3k) inhibitor attenuated cell proliferation and migration. however,inhibition of the soc channels failed to prevent egf-mediated erk 1/2 and akt phosphorylation. conclusions: our results showed that stim1,orai1,erk 1/2,and akt are key determinants of egf-mediated cell growth in arpe-19 cells. egf is a potent growth molecule that has been linked to the development of pvr,and therefore,stim1,orai1,as well as the mek/erk 1/2 and pi3k/akt pathways,might be potential therapeutic targets for drugs aimed at treating such disorders. © 2013 yang et al.; licensee biomed central ltd.
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کلیدواژه
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Orai1; Proliferative vitreoretinopathy; Retinal pigment epithelial cell; STIM1; Store-operated calcium channel
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آدرس
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department of medical genetics,college of medicine,kaohsiung medical university,kaohsiung,taiwan,department of ophthalmology,kaohsiung chang gung memorial hospital,chang gung university college of medicine,kaohsiung, Taiwan, department of medical genetics,college of medicine,kaohsiung medical university,kaohsiung, Taiwan, department of medical genetics,college of medicine,kaohsiung medical university,kaohsiung, Taiwan, department of medical laboratory science and biotechnology,college of medicine and life science,chung-hwa university of medical technology,tainan, Taiwan, department of medical genetics,college of medicine,kaohsiung medical university,kaohsiung, Taiwan, cancer center,kaohsiung medical university hospital,kaohsiung,taiwan,institute of clinical medicine,kaohsiung medical university,kaohsiung, Taiwan, center for resources,research and development,kaohsiung medical university,kaohsiung, Taiwan, institute of bioinformatics and biosignal transduction,college of bioscience and biotechnology,national cheng kung university,tainan, Taiwan, department of medical genetics,college of medicine,kaohsiung medical university,kaohsiung,taiwan,cancer center,kaohsiung medical university hospital,kaohsiung,taiwan,department of clinical pharmacy,school of pharmacy,taipei medical university,taipei,taiwan,department of pharmacy,taipei medical university,wanfang hospital,taipei,taiwan,master program for clinical pharmacogenomics and pharmacoproteomics,school of pharmacy,taipei medical university,taipei, Taiwan
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Authors
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