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Promotion of wound healing using adipose-derived stem cells in radiation ulcer of a rat model
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نویسنده
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huang s.-p. ,huang c.-h. ,shyu j.-f. ,lee h.-s. ,chen s.-g. ,chan j.y.-h. ,huang s.-m.
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منبع
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journal of biomedical science - 2013 - دوره : 20 - شماره : 1
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چکیده
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Background: wound healing is a complex biologic process that involves the integration of inflammation,mitosis,angiogenesis,synthesis,and remodeling of the extracellular matrix. however,some wounds fail to heal properly and become chronic. although some simulated chronic wound models have been established,an efficient approach to treat chronic wounds in animal models has not been determined. the aim of this study was to develop a modified rat model simulating the chronic wounds caused by clinical radiation ulcers and examine the treatment of chronic wounds with adipose-derived stem cells. results: sprague-dawley rats were irradiated with an electron beam,and wounds were created. the rats received treatment with adipose-derived stem cells (ascs),and a wound-healing assay was performed. the wound sizes after asc treatment for 3 weeks were significantly smaller compared with the control condition (p < 0.01). histological observations of the wound edge and immunoblot analysis of the re-epithelialization region both indicated that the treatment with ascs was associated with the development of new blood vessels. cell-tracking experiments showed that ascs were colocalized with endothelial cell markers in ulcerated tissues. conclusions: we established a modified rat model of radiation-induced wounds and demonstrated that ascs accelerate wound-healing. © 2013 huang et al.; licensee biomed central ltd.
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کلیدواژه
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Adipose-derived stem cells; Mesenchymal stem cells; Radiation ulcer; Wound model
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آدرس
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graduate institute of life sciences,national defense medical center,taipei 114, Taiwan, department of biochemistry,national,defense medical center,taipei 114, Taiwan, department of biology and anatomy,national defense medical center,taipei 114, Taiwan, department of pathology,tri-service general hospital,national defense medical center,taipei 114, Taiwan, graduate institute of life sciences,national defense medical center,taipei 114,taiwan,department of surgery,tri-service general hospital,national defense medical center,taipei 114, Taiwan, graduate institute of life sciences,national defense medical center,taipei 114,taiwan,department of microbiology and immunology,national defense medical center,taipei 114,taiwan,department of family and community medicine,tri-service general hospital,national defense medical center,taipei 114, Taiwan, graduate institute of life sciences,national defense medical center,taipei 114,taiwan,department of biochemistry,national,defense medical center,taipei 114, Taiwan
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Authors
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