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Up-regulation of S100A16 expression promotes epithelial-mesenchymal transition via Notch1 pathway in breast cancer
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نویسنده
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zhou w. ,pan h. ,xia t. ,xue j. ,cheng l. ,fan p. ,zhang y. ,zhu w. ,xue y. ,liu x. ,ding q. ,liu y. ,wang s.
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منبع
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journal of biomedical science - 2014 - دوره : 21 - شماره : 1
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چکیده
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Background: our previous studies demonstrated that s100a16 promotes adipogenesis and is involved in weight gain attenuation induced by dietary calcium. till now,the function of s100a16 in the breast cancer remains to be elucidated.results: in this study,we observed that s100a16 was expressed in higher levels in human breast cancer tissues compared with paired adjacent non-cancerous tissues. further examination showed that overexpression of s100a16 in mcf-7 cells could increase cell proliferation and colony formation. one major mechanistic change was that s100a16 was able to up-regulate the transcription factors notch1,zeb1,and zeb2,which had the capacities to directly repress the expression of epithelial markers e-cadherin and β-catenin but increase mesenchymal markers n-cadherin and vimentin,a characterized phenotype of epithelial-mensenchymal transition (emt). in addition to display with morphologic change,migration and invasion were increased in s100a16 over-expressed mcf-7 cells. importantly,knockdown of notch1 by specific sirna could reverse the emt induced by s100a16 overexpression,which confirmed that notch1 played a critical role in the process of emt induced by s100a16.conclusions: all together,our data indicated that s100a16 had a potential function to regulate some embryonic transcription factors to promote emt in breast cancer cells which may be an important target site for the therapy of breast cancer. © 2014 zhou et al.; licensee biomed central ltd.
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کلیدواژه
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Breast cancer; EMT; MCF-7; Notch1; S100A16
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آدرس
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department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of oncology,lombardi comprehensive cancer center,georgetown university,washington,dc 20057, United States, department of pathology,nanjing drum tower hospital,nanjing university medical school,321 zhongshan road,nanjing,210008, China, department of urology,zhongda hospital affiliated to southeast university,nanjing,210008, China, department of geratology,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of geratology,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China, department of breast surgery,first affiliated hospital with nanjing medical university,300 guangzhou road,nanjing,210029, China
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Authors
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