Japanese encephalitis virus replicon-based vaccine expressing enterovirus-71 epitope confers dual protection from lethal challenges

Background: to construct safer recombinant flavivirus vaccine,we exploited japanese encephalitis virus (jev) replicon-based platform to generate single-round infectious particles (srips) that expressed heterologous neutralizing epitope sp70 derived from enterovirus-71 (ev71). such pseudo-infectious virus particles,named srip-sp70,although are not genuine viable viruses,closely mimic live virus infection to elicit immune responses within one round of viral life cycle. results: we found that,besides gaining of full protection to thwart jev lethal challenge,female outbred icr mice,when were immunized with srip-sp70 by prime-boost protocol,could not only induce sp70-specific and igg2a predominant antibodies but also provide their newborns certain degree of protection against ev71 lethal challenge. conclusions: our results therefore exemplify that this vaccination strategy could indeed confer an immunized host a dual protective immunity against subsequent lethal challenge from jev or ev71. © 2015 huang et al.