Phage display as a promising approach for vaccine development

Bacteriophages are specific antagonists to bacterial hosts. these viral entities have attracted growing interest as optimal vaccine delivery vehicles. phages are well-matched for vaccine design due to being highly stable under harsh environmental conditions,simple and inexpensive large scale production,and potent adjuvant capacities. phage vaccines have efficient immunostimulatory effects and present a high safety profile because these viruses have made a constant relationship with the mammalian body during a long-standing evolutionary period. the birth of phage display technology has been a turning point in the development of phage-based vaccines. phage display vaccines are made by expressing multiple copies of an antigen on the surface of immunogenic phage particles,thereby eliciting a powerful and effective immune response. also,the ability to produce combinatorial peptide libraries with a highly diverse pool of randomized ligands has transformed phage display into a straightforward,versatile and high throughput screening methodology for the identification of potential vaccine candidates against different diseases in particular microbial infections. these libraries can be conveniently screened through an affinity selection-based strategy called biopanning against a wide variety of targets for the selection of mimotopes with high antigenicity and immunogenicity. also,they can be panned against the antiserum of convalescent individuals to recognize novel peptidomimetics of pathogen-related epitopes. phage display has represented enormous promise for finding new strategies of vaccine discovery and production and current breakthroughs promise a brilliant future for the development of different phage-based vaccine platforms. © 2016 the author(s).