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Improving the regenerative potential of olfactory ensheathing cells by overexpressing prostacyclin synthetase and its application in spinal cord repair
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نویسنده
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tsai m.-j. ,huang c.-t. ,huang y.-s. ,weng c.-f. ,shyue s.-k. ,huang m.-c. ,liou d.-y. ,lin y.-r. ,cheng c.-h. ,kuo h.-s. ,lin y. ,lee m.-j. ,huang w.-h. ,huang w.-c. ,cheng h.
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منبع
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journal of biomedical science - 2017 - دوره : 24 - شماره : 1
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چکیده
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Background: olfactory ensheathing cells (oec),specialized glia that ensheathe bundles of olfactory nerves,have been reported as a favorable substrate for axonal regeneration. grafting oec to injured spinal cord appears to facilitate axonal regeneration although the functional recovery is limited. in an attempt to improve the growth-promoting properties of oec,we transduced prostacyclin synthase (pgis) to oec via adenoviral (ad) gene transfer and examined the effect of oec with enhanced prostacyclin synthesis in co-culture and in vivo. prostacyclin is a vasodilator,platelet anti-aggregatory and cytoprotective agent. results: cultured oec expressed high level of cyclooxygneases,but not pgis. infection of adpgis to oec could selectively augument prostacyclin synthesis. when cocultured with either oec or adpgis-oec,neuronal cells were resistant to ogd-induced damage. the resulted oec were further transplanted to the transected cavity of thoracic spinal cord injured (sci) rats. by 6 weeks post-surgery,significant functional recovery in hind limbs occurred in oec or adpgis-oec transplanted sci rats compared with nontreated sci rats. at 10-12 weeks postgraft,adpgis-oec transplanted sci rats showed significantly better motor restoration than oec transplanted sci rats. futhermore,regenerating fiber tracts in the distal spinal cord stump were found in 40-60% of adpgis-oec transplanted sci rats. conclusions: enhanced synthesis of prostacyclin in grafted oec improved fiber tract regeneration and functional restoration in spinal cord injured rats. these results suggest an important potential of prostacyclin in stimulating oec therapeutic properties that are relevant for neural transplant therapies. © 2017 the author(s).
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کلیدواژه
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Gene transfer; Olfactory ensheathing glia; Prostacyclin; Spinal nerve regeneration; Transplantation
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آدرس
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neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217,taiwan,department of veterinary medicine,college of veterinary medicine,national chung hsing university,taichung,40227, Taiwan, department of veterinary medicine,college of veterinary medicine,national chung hsing university,taichung,40227, Taiwan, institute of biotechnology,national dong hwa university,hualien,97401, Taiwan, institute of biomedical sciences,academia sinica,taipei,11529, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217,taiwan,center for neural regeneration,neurological institute,taipei veterans general hospital,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217,taiwan,graduate institute of veterinary pathobiology,college of veterinary medicine,national chung hsing university,taichung,40227, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217,taiwan,department of applied chemistry,chaoyang university of technology,taichung,41349, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217,taiwan,center for neural regeneration,neurological institute,taipei veterans general hospital,taipei,11217, Taiwan, neural regeneration laboratory,center for neural regeneration,department of neurosurgery,neurological institute,taipei veterans general hospital,no. 322,shih-pai road,beitou district,taipei,11217,taiwan,center for neural regeneration,neurological institute,taipei veterans general hospital,taipei,11217,taiwan,institute and department of pharmacology,national yang-ming university,taipei,11221, Taiwan
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Authors
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