Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5]

Objective to verify the presence of anti-rotavirus serotype g9p[5] siga and the virus neutralization capacity of milk samples from brazilian women. methods siga antibody levels reactive to rotavirus g9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (elisa) using purified virus suspensions. the samples' capacity to neutralize rotavirus g9p[5] was analyzed using the ma-104 cells neutralization assay. results great individual variations were observed regarding the siga levels and neutralization titers,but all samples showed some g9p[5] neutralizing ability. a highly significant positive correlation was observed between antibody levels and neutralization titers. conclusions the high correlation between anti-rotavirus antibody levels and neutralizing capacity of the milk samples suggests a possible protective role of these antibodies against infection. these results also support the encouragement of the breast-feeding practice. © 2013 sociedade brasileira de pediatria.