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   IgG, IgA and IgE Reactivities to Sperm Antigens in Infertile Women  
   
نویسنده Brázdová Andrea ,Zídková Jarmila ,Peltre Gabriel ,Ulčová-Gallová Zdena
منبع jordan journal of biological sciences - 2012 - دوره : 5 - شماره : 2 - صفحه:85 -89
چکیده    Nowadays, world health organization (who) appoints infertility as a disease. still more couples are diagnosed with unexplained infertility, it reaches almost 20 % of all cases. immune system plays an important role: iso-immunization of women is possible by human sperm cells. for better understanding, it is necessary to detect and characterize antigens recognized by women immune system. our study is focused on female infertility and is based on the ejaculate sample from four healthy donors. antigens analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis and isoelectric focusing (followed by igg, iga and ige immunoblotting) were detected by forty-five sera from women who were diagnosed with fertility failure. ten children’s sera were used as negative controls. the immunodominant sperm antigens detected by igg antibodies were 68 and 123 kda proteins with pi from 6.60 to 6.62, respectively. concerning mucosal immunity the most frequently recognized sperm iga-binding protein reached 130 kda and pi 6.9, 7.4, 9.5. this study did not prove the association of ige antibodies with unexplained infertility in women
کلیدواژه Human sperm ,unexplained infertility ,polyacrylamide gel electrophoresis ,isoelectric focusing ,immunoblotting
آدرس Institute of Chemical Technology Prague, Department of Biochemistry and Microbiology, Czech Republic, Institute of Chemical Technology Prague, Department of Biochemistry and Microbiology, Czech Republic, École Supérieure de Physique et de Chimie Industrielles de la Ville de Paris, Laboratoire Sciences Analytiques, Bioanalytique et Miniaturisaton (LSABM), France, Charles University, Faculty Hospital, Department of Obstetrics and Gynecology, Czech Republic
 
     
   
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