Assessment of antigenotoxic effect of nanoselenium and metformin on diabetic rats

The present study was conducted to investigate the protective role of selenium nanoparticles on type-2 diabetes mellitus (t2dm) in male rats treated with metformin drug compared to the control. chromosomal aberrations,dna fragmentation,micronucleus tests as well as comet assay were carried out. rats were divided into four groups: group1,normal control in which the rats were received normal saline solution,group 2,control diabetic group in which the diabetic rats were induced using 45mg/kg body weight streptozotocin (stz). group 3,metformin treated group in which the diabetic rats were treated with a standard oral hypoglycemic agent,metformin (100 mg/kg body weight (b.wt) while,group 4,selenium nanoparticles (senps) treated group,in which the rats were treated with nanoselenium stabilized in liposome (0.1 mg/kg b. wt) orally plus metformin. rats were sacrificed after 21 days following the last injection. the results demonstrated that diabetes caused increase in chromosomal aberrations,micronucleus formation,dna fragmentation as well as dna damage using comet assay,but these parameters decrease in treated animals with metformin. in addition,metformin + nanoselenium combined treatment caused decreased in all studied parameters relative to metformin alone. in conclusion,it appeared that senps combined with metformin have antidiabetic effect in experimental model of t2dm. this effect could be ascribed to their ability in decreasing frequencies of chromosomal aberrations,decreasing the number of micronucleus as well as decreasing the percentages of dna damage. thus,senps may benefit in clinical purposes especially in enhancing the effect of t2dm. © 2017 jordan journal of biological sciences.